The Effect of c-fos on Acute Myocardial Infarction and the Significance of Metoprolol Intervention in a Rat Model
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ORIGINAL PAPER
The Effect of c-fos on Acute Myocardial Infarction and the Significance of Metoprolol Intervention in a Rat Model Song Zhang • Meiqi Zhang • Steven Goldstein Yigang Li • Junbo Ge • Ben He • George Ruiz
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Published online: 5 October 2012 Ó Springer Science+Business Media New York 2012
Abstract Over-expression of c-fos may play a role in some diseases. Research pertaining to the expression of c-fos in acute myocardial ınfarction (AMI) is rare, and the detailed role of c-fos in AMI has not been reported. Therefore, the purpose of this project was to elucidate the detailed effect of c-fos on AMI rats and evaluate the effect of a metoprolol intervention. An AMI rat model was established for the purposes of this study. The expression of c-fos in AMI was evaluated via immunohistochemical analysis and in situ hybridization. Simultaneously, we investigated the effect of c-fos on AMI rats via medicinal treatment with c-fos monoclonal antibody, isoproterenol, and metoprolol. Positive c-Fos protein expression and c-fos mRNA expression in cardiomyocytes were increased at 1, 3, 7, and 10 days after ligation in AMI rats compared with
S. Zhang Y. Li (&) Department of Cardiovascular Diseases, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, 1665 Kongjiang Road, Shanghai 200092, China e-mail: [email protected] M. Zhang Emergency Department, People’s Hospital of Zhejiang, 158 Shangtang Road, Hangzhou 310014, Zhejiang, China S. Goldstein G. Ruiz Department of Cardiology, Washington Hospital Center, 110 Irving Street, Washington, DC 20010, USA J. Ge Department of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China B. He Department of Cardiovascular Diseases, Eastern District of Renji Hospital, Shanghai Jiaotong University, 1630 Dongfang Road, Shanghai 200127, China
a sham-operated group. Peak expression occurred at 3 days after ligation. The weight percentage fraction of infarct size was decreased in rats treated with c-fos monoclonal antibody compared with the control normal saline treatment group. The weight percentage fraction of infarction size was increased after c-fos was increased via the administration of isoproterenol. c-Fos protein expression and the infarct size in rats treated with metoprolol were also decreased compared with the control normal saline treatment group. The results showed that c-fos expression rapidly increased after coronary ligation; c-fos plays an important role in myocardial lesions and is likely to be involved in the pathogenesis of AMI as well. Metoprolol can inhibit the expression of c-fos and has a positive therapeutic effect on rats after AMI; the involvement effect of metoprolol on myocardial infarction might be correlated with its effect on the inhibition of c-fos. Keywords Acute myocardial ınfarction c-fos Rat Effect Metoprolol
Introduction The c-Fos protein encoded by Fos (v-fos FBJ murine osteosarcoma viral oncogene homolog) is a regulatory protein that has a basic leucine-zipper region fo
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