The Effect of miR-155 on DNA Damage in Mesenchymal Stem Cells
- PDF / 826,113 Bytes
- 8 Pages / 612 x 792 pts (letter) Page_size
- 17 Downloads / 134 Views
he Effect of miR-155 on DNA Damage in Mesenchymal Stem Cells Mohammad Shokouhiana, Minoo Shahidia, *, and Mohammad Ali Gholampourb aDepartment
of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran b Department Department, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran *e-mail: [email protected] Received September 19, 2019; revised February 28, 2020; accepted March 3, 2020
Abstract—Considering the crucial role of miR-155 in DNA repair regulation, the aim of this study was to evaluate the effect of miR-155 on the expression of DNA repair factors involved in single- and double-strand DNA breaks (SSBs and DSBs) and mismatch repair (MMR) in mesenchymal stem cells (MSCs). MSCs were isolated from the bone marrow of a healthy individual, and then confirmed by their adipogenic/osteogenic differentiation and flow cytometric analysis of surface markers of MSCs (CD105, CD90, and CD73). MSCs transfection by green fluorescent protein (GFP) plasmid bearing miR-155 was verified by fluorescent microscope, and was measured via analyzing the percentage of transfected cells compared to non-transfected by flow cytometry. The expression of miR-155 and mRNAs related to DNA repair response system, i.e. XRCC1, XRCC5, XRCC6, and RAD51, were assessed by real-time PCR. Overexpression of miR-155 in MSCs was significant compared to control (p = 0.034). Increase in the expression of DNA repair genes, including XRCC1, XRCC6, XRCC5, and RAD51 was not significant (p = 0.066, 0.108, 0.092, 0.631, and 0.262, respectively). In conclusion, although the expression of DNA repair genes by miR-155 overexpression was not significant, it has the potential to affect DNA repair genes expression. Keywords: DNA damage, Mesenchymal stem cells, miR-155 DOI: 10.1134/S1990519X20050077
INTRODUCTION MicroRNAs (miRNAs), a group of small noncoding RNAs, regulate gene expression at the posttranscription level via either enhancing the degradation of mRNA or inhibiting its translation, and have role in various cell processes, including proliferation, apoptosis, and differentiation (Yangling Wang et al., 2018; Zhao et al., 2019). Many studies have revealed the role of miRNAs in the regulation of various types of DNA repair proteins, on which the maintenance of cell genomic stability depends (He et al., 2016). Modulation of miRNAs could have various effects on these proteins; for instance, miR-19 overexpression disturbs the repair of double strand breaks (DSBs) through downregulating CtIP, which mediates DNA-end restriction of DSBs during the repair by homologous recombination mechanism (Xia et al., 2019). In another study, it was reported that miR-126 downregulation is a key casual factor in hepatocellular carcinoma (HCC) which negatively affect PLKAbbreviarions: BER—base excision repair, BM—bone marrow, DDR—DNA damage response, DSBs—double-strand breaks, HCC—hepatocellular carcinoma, HSCs—hematopoietic stem cells, miR—microRNAs, MMR—mismatch repair, MSCs— mesenchymal stem cells, NER—nucle
Data Loading...
