The effects of treatments with multistrain probiotics on serum aminotransferases, AST-ALT ratio, body weight and surviva
- PDF / 463,849 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 72 Downloads / 162 Views
ORIGINAL ARTICLE
The effects of treatments with multistrain probiotics on serum aminotransferases, AST-ALT ratio, body weight and survivability scores of rats experimentally infected with Trypanosoma brucei Chukwuemeka Calistus Okolo 1 & Chinelo Nnenna Uju 2 & Solomon David Chibuzor 3 & Kelechi Jessica Ezema 4 & Oscar Dahenji Kolndadacha 5 & Amina Ibrahim 6 & Chinwe Justina Aronu 2 Received: 6 August 2020 / Accepted: 16 October 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract Probiotics can modulate gut-liver axis. Effects of administration of multistrain probiotics (MP) on liver damage and overall health of trypanosomic rats were investigated. Thirty male rats randomly assigned to five groups A–E (n = 6), and MP containing Bifidobacterium BB-12, Lactobacillus delbrueckii LBY-27, Lactobacillus acidophilus LA-Z, Streptococcus thermophilus STY-31 and Lactobacillus paracasei LC-01 were used for supplementation. From day 0 post-supplementation (PS), rats in groups A–C received 1 × 109, 5 × 109 and 10 × 109 CFUs of MP daily and respectively. Groups D and E received no MP supplementation being the infected and uninfected controls respectively. On day 7PS, groups A–D were challenged with approximately 1 × 106 Trypanosoma brucei intraperitoneally. On days 0, 7 and 16, PS, serum alanine and aspartate aminotransferases (ALT and AST) were determined spectrophotometrically, while parasitaemia, bodyweight and mortality were monitored. By day 7, PS groups BD had similar ALT activities and AST-ALT ratios, while all groups had similar AST activities. On day 16, PS all infected groups (A–D) had similar and significantly (p < 0.05) lower AST activities and AST-ALT ratio compared to the uninfected control, while ALT activities were similar and significantly elevated in groups A-D. Logarithmic parasitaemia levels and AST-ALT ratio showed random moderate negative correlation (r = − 0.390; p = 0.059). No significant variations in bodyweight were seen, and all infected groups had 100% mortality by day 27 post-supplementation. Supplementation with MP was innocuous to liver function in naïve rats, but failed to improve liver-function parameters, bodyweight or survivability of trypanosomic rats. Keywords Probiotics . Aminotransferases . AST-ALT ratio . Trypanosomosis . Bodyweight
* Chukwuemeka Calistus Okolo [email protected]; [email protected] Chinelo Nnenna Uju [email protected] Solomon David Chibuzor [email protected] Kelechi Jessica Ezema [email protected] Oscar Dahenji Kolndadacha [email protected]
1
Department of Veterinary Medicine, University of Nigeria Nsukka, Nsukka, Enugu State 410001, Nigeria
2
Department of Animal Health and Production, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria
3
Department of Veterinary Pharmacology Physiology and Biochemistry, University of Nigeria Nsukka, Nsukka, Enugu State, Nigeria
4
Empire Veterinary Clinic Gowon Estate, Lagos, Lagos State, Nigeria
5
Department of Animal Health and Production, Fac
Data Loading...
