The Molecular Detection of Corynespora Cassiicola on Cucumber by PCR Assay Using DNAman Software and NCBI
Objective: to establish a quick molecular detection method in Beijing, which can prevent the occurrence of cucumber target spot disease from the source. Methods and results: The DNA band of Corynespora cassiicola had been obtained by PCR and sequenced. Us
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Abstract. Objective: to establish a quick molecular detection method in Beijing, which can prevent the occurrence of cucumber target spot disease from the source. Methods and results: The DNA band of Corynespora cassiicola had been obtained by PCR and sequenced. Using DNAman software and NCBI database to analyze the sequence, the results showed that the obtained DNA was that of Corynespora cassiicola on Cucumber. A specific primers CCC1/CCC2 were obtained by DNAman software and NCBI database. It was also proved to can be used to distinguish Corynespora cassiicola from other pathogenic fungi using DNAman software and NCBI database. Keywords: Molecular detection Corynespora cassiicola DNAman NCBI
1 Introduction A cucumber target spot disease caused by Corynespora cassiicola (Berk. & Curt.) occurs serious damage. The leaves are the main victims and in severe cases the pathogenic fungi can spread to the petioles and even to the vine. Both on the top and back of leaves, large and small necrotic lesions can be formed and there is one white bull’s eye on the center and even cause leaves dry by the humidity lesion. With the development of modern agriculture, in 2012 the agricultural facilities are expected to develop 35 acres. Facilities agriculture has been positioned as a main direction in the development of agriculture of Beijing. Cucumber, as an important vegetable in Beijing has been more attention in pest and disease control work. Spot disease caused by Corynespora cassiicola in cucumber is very serious and rapid progression in recent years and has caused more and more damages in China. Its occurrence was the growing trend and is causing serious economic losses to farmers from 2005 to 2010. The current study shows that seed infection is an important spread way of cucumber target spot disease (Corynespora cassiicola) and in seeds markets the parameters related to purity, germination rate, moisture content are often detected. But the seed infection is often ignored, which often results in the occurrence of target leaf spot disease caused by Corynespora cassiicola in cucumber and results in late serious impact on growth and yield. Traditional detection methods are time-consuming, low sensitivity, susceptible to the interference of man-made and environmental factors, etc. so it is necessary to establish a fast, easy and accurate detection method, provide the basis for early diagnosis and prevention of disease, which can prevent the occurrence of cucumber Corynespora © IFIP International Federation for Information Processing 2016 Published by Springer International Publishing AG 2016. All Rights Reserved D. Li and Z. Li (Eds.): CCTA 2015, Part II, IFIP AICT 479, pp. 248–258, 2016. DOI: 10.1007/978-3-319-48354-2_26
The Molecular Detection of Corynespora Cassiicola on Cucumber
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cassiicola from the source. And if the seed can be resolved through physical measures, it can be effective technical measures to the comprehensive prevention and control of the target leaf spot in cucumber. This study aims to establish a
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