Complete genome sequence of a novel mycovirus isolated from the phytopathogenic fungus Corynespora cassiicola in China
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ANNOTATED SEQUENCE RECORD
Complete genome sequence of a novel mycovirus isolated from the phytopathogenic fungus Corynespora cassiicola in China Yanfen Wang1 · Hang Zhao1 · Caiying Xue1 · Chao Xu1 · Yuehua Geng1 · Rui Zang1 · Yuxia Guo3 · Haiyan Wu2 · Meng Zhang1 Received: 24 March 2020 / Accepted: 1 June 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel mycovirus, named “Corynespora cassiicola bipartite mycovirus 1” (CcBV1), was isolated from a phytopathogenic fungus, Corynespora cassiicola, the causal agent of rubber leaf fall disease. The nucleotide sequence of the complete genome of CcBV1, which consists of two double-stranded RNA (dsRNA) segments, was determined. The first dsRNA is 2,002 bp in length and contains a single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) (69 kDa), while the second is 1,738 bp in length and contains a single ORF encoding a hypothetical protein of unknown function, with an approximately molecular weight of 36 kDa. The amino acid sequences of the both deduced proteins are most similar (58.9% and 45.1% identity, respectively) to those of Cryphonectria parasitica bipartite mycovirus 1 (CpBV1). Phylogenetic analysis indicated that CcBV1 clusters together with CpBV1 and other unassigned dsRNA mycoviruses. To the best of our knowledge, this represents the first report of a mycovirus infecting C. cassiicola. The majority of mycoviruses have genomes composed of double-stranded RNA (dsRNA), positive-sense singlestranded RNA (+ssRNA), negative-sense ssRNA (-ssRNA), or circular ssDNA [1–3]. Mycoviruses can infect all major groups of plant-pathogenic fungi [4, 5], but most of them do not cause any obvious symptoms in fungi, with only a few being reported to induce abnormality in their hosts, such as an altered growth rate or hypovirulence [6, 7]. Mycoviruses associated with hypovirulence are of great interest, as they Handling Editor: Robert H. A. Coutts.
have the potential for use as biocontrol agents against fungal diseases [8]. Moreover, many novel viruses have greatly advanced our understanding of the virus world [9–11]. The plant-pathogenic fungus Corynespora cassiicola has a wide host range and can infect plants belonging to more than 530 species. Serious outbreaks of Corynespora leaf spot have been documented in many crops, including rubber tree and various vegetable species, which have had a dramatic impact on crop production. To date, no mycoviruses have been reported to infect this important fungus. The current study is therefore of great interest, as it details
* Haiyan Wu [email protected]
Rui Zang [email protected]
* Meng Zhang [email protected]
Yuxia Guo [email protected]
Yanfen Wang [email protected]
1
Hang Zhao [email protected]
College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan, China
2
Caiying Xue [email protected]
Centre for Modern Experimental Technology, Henan Agricultural University, Zhengzhou 450002, Henan, China
3
College of Animal and Veterinary S
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