The nature and features of organization of reserve polysaccharides in three Pelomyxa species (Archamoebea, Pelobiontida)
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ORIGINAL ARTICLE
The nature and features of organization of reserve polysaccharides in three Pelomyxa species (Archamoebea, Pelobiontida) Ludmila Chistyakova 1 & Natalia Bezborodkina 1 & Mariia Berdieva 2 & Anton Radaev 3 & Andrew Goodkov 2 Received: 2 June 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The nature and features of organization of reserve polysaccharides in three species of the genus Pelomyxa—P. palustris, P. belevskii, and P. stagnalis—were studied using light and transmission electron microscopy. We applied the periodic acidSchiff reaction that is a highly selective method for detecting glycogen. The fluorescent dye auramine-SO2 (Au-SO2) was used as a Schiff-type reagent. The densely packed aggregates of glycogen that form the morphologically differentiated organelle-like bodies are revealed in the cytoplasm in all studied species. The organization of these bodies is characterized by the speciesspecific features, while in most cases, their size and number in the cells vary depending on the season of the year. Although in all the cases we studied, these bodies do not have their own boundary membrane, in fact, they are surrounded by membranous structures. These structures differ in a variety of Pelomyxa species. We concluded that there are two groups of species in the genus Pelomyxa. The first one includes organisms containing glycogen structures in the cytoplasm (P. palustris, P. belevskii, P. stagnalis, P. binucleata, P. corona, P. secunda). No inclusions resembling glycogen bodies were found in P. flava, P. paradoxa, P. gruberi, and P. prima that form the second group. Keywords Electron microscopy . Glycogen bodies . Fluorescent microscopy . Light microscopy . Pelomyxa
Introduction The genus Pelomyxa currently includes 10 species of freeliving freshwater amoeboid protists inhabiting oxygenpoor ecotopes. Some species can reach up to 4 mm in length. Pelomyxa species are predominantly multinuclear organisms; most of them have numerous non-functioning flagella randomly distributed on the cell surface (Griffin 1988; Frolov 2011; Chistyakova et al. 2013; Ptáčková et al. 2013). The cytoplasm is highly vacuolated due to the presence of numerous so-called “structural” vacuoles in the cells of most of Pelomyxa species. These vacuoles Handling Editor: Georg Krohne * Ludmila Chistyakova [email protected] 1
Zoological Institute Russian Academy of Sciences, Universitetskaya Emb. 1, 199034 St. Petersburg, Russia
2
Institute of Cytology Russian Academy of Sciences, 194064 St. Petersburg, Russia
3
Saint-Petersburg State University, 199034 St. Petersburg, Russia
are inherently the derivatives of endoplasmic reticulum (Andresen et al. 1968; Goodkov and Seravin 1991; Chistyakova et al. 2013). Pelomyxids have no mitochondria and Golgi dictyosomes. They have numerous prokaryotic endocytobionts belonging to several morphologically different types (Andresen et al. 1968; Whatley 1976; Chistyakova et al. 2016; etc.). A characteristic feature of some Pelomyxa sp
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