The Saccharomyces cerevisiae flavodoxin-like proteins Ycp4 and Rfs1 play a role in stress response and in the regulation
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ORIGINAL PAPER
The Saccharomyces cerevisiae flavodoxin-like proteins Ycp4 and Rfs1 play a role in stress response and in the regulation of genes related to metabolism Fernando Cardona • Helena Orozco • Sylvie Friant • Agustı´n Aranda • Marcellı´ del Olmo
Received: 9 December 2010 / Revised: 15 February 2011 / Accepted: 11 March 2011 / Published online: 27 March 2011 Ó Springer-Verlag 2011
Abstract SPI1 is a gene whose expression responds to many environmental stimuli, including entry into stationary phase. We have performed a screening to identify genes that activate SPI1 promoter when overexpressed. The phosphatidylinositol-4-phosphate 5-kinase gene MSS4 was identified as a positive activator of SPI1. Another SPI1 transcriptional regulator isolated was the flavodoxin-like gene YCP4. YCP4 and its homolog RFS1 regulate the expression of many genes during the late stages of growth. The double deletion mutant in YCP4 and its homolog RFS1 has an impact on gene expression related to metabolism by increasing the expression of
genes involved in hexose transport and glycolysis, and decreasing expression of genes of amino acid metabolism pathways. Genes related to mating and response to pheromone show a decreased expression in the double mutant, while transcription of genes involved in translational elongation is increased. Deletion of these genes, together with the third member of the family, PST2, has a complex effect on the stress response. For instance, double mutant ycp4Drfs1D has an increased response to oxidative stress, but a decreased tolerance to cell-damaging agent SDS. Additionally, this mutation affects chronological aging and slightly increases fermentative capacity.
Communicated by Axel Braghage.
Keywords S. cerevisiae Stress Flavodoxin-like YCP4 RFS1
Electronic supplementary material The online version of this article (doi:10.1007/s00203-011-0696-7) contains supplementary material, which is available to authorized users.
Introduction
F. Cardona H. Orozco A. Aranda (&) Departamento de Biotecnologı´a, Instituto de Agroquı´mica y Tecnologı´a de Alimentos (CSIC), Av. Agustı´n Escardino 7, 46980 Paterna, Spain e-mail: [email protected] F. Cardona H. Orozco M. del Olmo Departament de Bioquı´mica i Biologia Molecular, Universitat de Vale`ncia, Burjassot, Spain S. Friant Membrane trafficking and lipid signaling, Ge´ne´tique Mole´culaire, Ge´nomique et Microbiologie, UMR7156 (CNRS-UdS), Strasbourg, France Present Address: F. Cardona Unitat de Gene`tica Molecular, Institut de Biomedicina de Vale`ncia, Vale`ncia, Spain
In media with glucose as the sole carbon source and an excess of nutrients, biomass grows exponentially, metabolism is fermentative, and respiratory metabolism genes are repressed. When levels of the fermentable carbon source decrease, the cell enters into a transition phase between fermentative and respiratory metabolism, called the diauxic shift. This is followed by the post-diauxic growth phase, characterized by respiratory metabolism, transcription of the genes repressed b
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