The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition an
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The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition and growth performance of peanut (Arachis hypogaea L.) in saline alkali soil§ Dunwei Ci1†, Zhaohui Tang2†, Hong Ding1, Li Cui3, Guanchu Zhang1, Shangxia Li1, Liangxiang Dai1, Feifei Qin1, Zhimeng Zhang1, Jishun Yang1*, and Yang Xu1* 1 Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, Shandong 266100, P. R. China 2 Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Shandong Key Lab. of Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, P. R. China 3 Shandong Provincial Crop Germplasm Resource Centre, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China
(Received Jun 22, 2020 / Revised Oct 12, 2020 / Accepted Oct 12, 2020)
Peanut (Arachis hypogaea. L) is an important oil seed crop. Both arbuscular mycorrhizal fungi (AMF) symbiosis and calcium (Ca2+) application can ameliorate the impact of saline soil on peanut production, and the rhizosphere bacterial communities are also closely correlated with peanut salt tolerance; however, whether AMF and Ca2+ can withstand high-salinity through or partially through modulating rhizosphere bacterial communities is unclear. Here, we used the rhizosphere bacterial DNA from saline alkali soil treated with AMF and Ca2+ alone or together to perform high-throughput sequencing of 16S rRNA genes. Taxonomic analysis revealed that AMF and Ca2+ treatment increased the abundance of Proteobacteria and Firmicutes at the phylum level. The nitrogenfixing bacterium Sphingomonas was the dominant genus in these soils at the genus level, and the soil invertase and urease activities were also increased after AMF and Ca2+ treatment, implying that AMF and Ca2+ effectively improved the living environment of plants under salt stress. Moreover, AMF combined with Ca2+ was better than AMF or Ca2+ alone at altering the bacterial structure and improving peanut growth in saline alkali soil. Together, AMF and Ca2+ applications are conducive to peanut salt adaption by regulating the bacterial community in saline alkali soil. Keywords: Arachis hypogaea L., 16S rRNA, rhizosphere bacterial community, saline alkali soil, AMF, Ca2+ †
These authors contributed equally to this work. *For correspondence. (Y. Xu) E-mail: [email protected] / (J.S. Yang) E-mail: [email protected] / (Y. Xu and J.S. Yang) Tel.: +86-532-87610802; Fax: +86-532-87610802 § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright ⓒ 2021, The Microbiological Society of Korea
Introduction Peanut (Arachis hypogaea L.) is an important oil and cash crop cultivated worldwide (Xu et al., 2020a). Irrational agricultural irrigation practices and an increasing population have aggravated the conflict between grain and oil arable land. Due to the limited arable land and the requirement for developing regional agriculture, peanuts have been cultivated in the sal
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