The Topical Cream Produced from Phycocyanin of Spirulina platensis Accelerates Wound Healing in Mice Infected with Candi
- PDF / 936,567 Bytes
- 7 Pages / 612 x 792 pts (letter) Page_size
- 13 Downloads / 189 Views
he Topical Cream Produced from Phycocyanin of Spirulina platensis Accelerates Wound Healing in Mice Infected with Candida albicans N. Zamania, M. Fazilatia, H. Salavatia, M. Izadia, and M. Koohi-Dehkordib, * aDepartment
of Biology, Payame Noor University, Tehran, 19395-3697 Iran Department of Agricultural Sciences, Payame Noor University, Tehran, 19395-3697 Iran *e-mail: [email protected]
b
Received January 21, 2020; revised March 5, 2020; accepted April 22, 2020
Abstract—Application of natural colors in food and medicine, due to toxic effects of synthetic colors has been considered recently. Phycocyanin is regarded as the most preferred phycobilin pigment of cyanobacteria Spirulina because it has medical and medicinal features. In current study, phycocyanin was extracted and purified from Spirulina platensis using 5 freezing/thawing cycles, ultrasonic disruption, ammonium sulphate precipitation and dialysis. Purified phycocyanin was tested by UV-spectrophotometrically and using FT-IR and HPLC methods. The antifungal effect of purified phycocyanin was evaluated versus Candida albicans on sabouraud dextrose agar plates, and maximum anticandidal activity was revealed in the range from 20 to 25 mg/mL of phycocyanin. Making sure of phycocyanin’s antifungal effect, the efficacy of its produced cream (using 1.5 and 3% of phycocyanin compared to control) was investigated on mice infected with C. albicans. Based on obtained results, those groups of mice treated by formulation containing phycocyanin improved much sooner than control group. Keywords: Antifungal activity, Candida albicans, cream, phycocyanin, Spirulina platensis DOI: 10.1134/S0003683820050166
In recent years, clinical importance of systemic candidiasis has been increased for immunocompromised patients with resistance to conventional antifungal treatment [1, 2]. Candida albicans is a common opportunistic pathogen capable of attacking deep limbs in definite situations [3]. Patients with deficiency in cell-mediated immunity are sensitive to mucocutaneous, but not to systemic conditions. Systemic infections are generally associated with neutropenia and connate deficiencies. Both neutrophils and macrophages have proved to be in the first line of advocacy versus systemic Candida infections. Biological respond modulators can be helpful in improving host immune response (Mashihi and Lange, 1990; Gaforio et al., 2002). In prior studies, bacterial lipopolysaccharides, carrageenan, fungal β-glucan, pigments and Spirulina extracts have been utilized to activate macrophages against candidiasis [4–7]. Recently, search for innate compositions like cyanobacteria with antimicrobial effect has gained significance because of rising global interest to warning for infection via antibiotic-resistance microorganisms [8]. It is commonly believed that compositions made naturally are more easily biodegradable than those made artificially, and, therefore, are environmentally acceptable [9]. Spirulina is a member of the cyanobacteria which is used as a dietary supplement. This kind o
Data Loading...
