Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense , involved in ovary maturation
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ORIGINAL ARTICLE
Three neuroparsin genes from oriental river prawn, Macrobrachium nipponense, involved in ovary maturation Hui Qiao1 · Yiwei Xiong1 · Sufei Jiang1 · Wenyi Zhang1 · Lei Xu · Shubo Jin1 · Yongsheng Gong1 · Yan Wu1 · Hongtuo Fu1,2 Received: 11 August 2020 / Accepted: 28 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract In this study, we identified three neuroparsin (NP) genes in Macrobrachium nipponense: Mn-NP1, Mn-NP2, and Mn-NP3, encoding 99, 100, and 101 amino acid proteins, respectively. Multiple sequence alignments showed that these genes contained 12 cysteine residues, of which 11 were at conserved positions. The total sequence identity between the genes was 47.5%, and they showed a high degree of sequence identity (> 54% similarity) with other crustacean genes. Phylogenetic tree analysis showed that Mn-NPs were clustered at different branches, indicating that Mn-NPs may have different functions. Tissue distribution data revealed that the three genes were present in males and females during the breeding and nonbreeding season, but their expression patterns differed. Mn-NP1 was highly expressed in the breeding season, in the male testis, and highly expressed in the nonbreeding season, in the female ovary. Mn-NP3 exhibited biased female expression in the breeding and nonbreeding season, with dominant expression in the ovary. All Mn-NPs were detected during embryo development, but with different expression patterns. These data indicated that Mn-NP1 may function during embryonic development, and that Mn-NP2 may be expressed during early embryo cell division, and late larval development. Mn-NP3 expression patterns reflected maternal inheritance, and may be associated with ovarian maturation. These expression data suggested that Mn-NP1 and Mn-NP2 are negatively correlated with ovarian development, with inhibition roles during this development. Mn-NP3 may be involved in vitellogenesis. Keywords Macrobrachium nipponense · Neuroparsin · Ovary maturation · Reproduction
Introduction Neuroparsins (NP) are neuroendocrine polypeptides first discovered in the migratory locust, Locusta migratoria. They encode 97–106 amino acid proteins, with predicted signal peptides and mature peptides of 25–29 and 72–77 amino acids, respectively (Girardie et al. 1989). The NP structure is similar to the N-terminal domain of the vertebrate insulin Hui Qiao and Yiwei Xiong have contributed equally to this work and should be considered co-first authors. * Hongtuo Fu [email protected] 1
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, People’s Republic of China
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, People’s Republic of China
2
growth factor-binding protein, and may play important role in insulin-associated peptide signaling pathways during growth and development, by binding to endogenous insulin-like related peptides (Bad
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