Identification of a novel germ cell marker MnTdrd from the oriental river prawn Macrobrachium nipponense
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ORIGINAL ARTICLE
Identification of a novel germ cell marker MnTdrd from the oriental river prawn Macrobrachium nipponense Yao-Ting Dong 1,2,3,4 & Hai-Yang Feng 1,2,3,4 & Xiao-Qing Tian 1,2,3,4 & Qi-Liang Wang 1,2,3,4 & Shu-Fang Zhang 1,2,3,4 & Ke-Yi Ma 1,2,3,4 & Gao-Feng Qiu 1,2,3,4 Received: 9 August 2020 / Accepted: 4 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Germ cell-specific genes play an important role in establishing the reproductive system in sexual organisms and have been used as valuable markers for studying gametogenesis and sex differentiation. Previously, we isolated a vasa transcript as a germ cell marker to trace the origin and migration of germ cells in the oriental river prawn Macrobrachium nipponense. Here, we identified a new germ cell-specific marker MnTdrd RNA and assessed its temporal and spatial expression during oogenesis and embryogenesis. MnTdrd transcripts were expressed in high abundance in unfertilized eggs and embryos at cleavage stage and then dropped significantly during late embryogenesis, suggesting that MnTdrd mRNA is maternally inherited. In situ hybridization of ovarian tissue showed that MnTdrd mRNA was initially present in the cytoplasm of previtellogenic oocyte and localized to the perinuclear region as the accumulation of yolk in vitellogenic oocyte. Whole-mount in situ hybridization of embryos showed that MnTdrd-positive signals were only localized in one blastomere until 16-cell stage. In the blastula, there were approximately 16 MnTdrd-positive blastomeres. During embryonized-zoea stage, the MnTdrd-positive cells aggregated as a cluster and migrated to the genital rudiment which would develop into primordial germ cells (PGCs). The localized expression pattern of MnTdrd transcripts resembled that of the previously identified germ cell marker vasa, supporting the preformation mode of germ cell specification. Therefore, we concluded that MnTdrd, together with vasa, is a component of the germ plasm and might have critical roles in germ cell formation and differentiation in the prawn. Thus, MnTdrd can be used as a novel germ cell marker to trace the origin and migration of germ cells. Keywords Oriental river prawn . Germline specification . Tdrd . Germ cell marker
Introduction Yao-Ting Dong, Hai-Yang Feng, and Xiao-Qing Tian are the co-first authors. Communicated by Mark Q. Martindale * Gao-Feng Qiu [email protected] 1
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China
2
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
3
Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
4
College of Fisheries and Life Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong New Area, Shanghai 201306, China
Primordial germ cells (PGCs), as progenitor of germ cells, are specified during early development either by induction of so
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