Tissue engineering rib with the incorporation of biodegradable polymer cage and BMSCs/decalcified bone: an experimental
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Tissue engineering rib with the incorporation of biodegradable polymer cage and BMSCs/decalcified bone:an experimental study in a canine model Journal of Cardiothoracic Surgery 2013, 8:133
doi:10.1186/1749-8090-8-133
Hua Tang ([email protected]) Bin Wu ([email protected]) Xiong Qin ([email protected]) Lu Zhang ([email protected]) Jim Kretlow ([email protected]) Zhifei Xu ([email protected])
ISSN Article type
1749-8090 Research article
Submission date
3 December 2012
Acceptance date
13 May 2013
Publication date
20 May 2013
Article URL
http://www.cardiothoracicsurgery.org/content/8/1/133
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© 2013 Tang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Tissue engineering rib with the incorporation of biodegradable polymer cage and BMSCs/decalcified bone:an experimental study in a canine model Hua Tang1,† Email: [email protected] Bin Wu1,† Email: [email protected] Xiong Qin1 Email: [email protected] Lu Zhang2 Email: [email protected] Jim Kretlow3 Email: [email protected] Zhifei Xu1* * Corresponding author Email: [email protected] 1
Department of Thoracic and Cardiovascular Surgery, Shanghai Changzheng Hospital, The Second Military Medical University, No.415 Fengyang Road, Shanghai 200003, China 2
Shanghai Key Laboratory of Tissue Engineering, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China 3
†
Institute of Biosciences and Bioengineering, Rice University, Rice, USA
Equal contributors.
Abstract Background The reconstruction of large bone defects, including rib defects, remains a challenge for surgeons. In this study, we used biodegradable polydioxanone (PDO) cages to tissue engineer ribs for the reconstruction of 4cm-long costal defects.
Methods PDO sutures were used to weave 6cm long and 1cm diameter cages. Demineralized bone matrix (DBM) which is a xenograft was molded into cuboids and seeded with second passage bone marrow mesenchymal stem cells (BMSCs) that had been osteogenically induced. Two
DBM cuboids seeded with BMSCs were put into the PDO cage and used to reconstruct the costal defects. Radiographic examination including 3D reconstruction, histologic examination and mechanical test was performed after 24 postoperative weeks.
Results All the experimental subjects survived. In all groups, the
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