Toxicity Effect of Cancer Cell Labeled with Visible Luminescent Nanocrystalline Silicon Particles and Visualization Obse
- PDF / 272,672 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 45 Downloads / 128 Views
1145-MM04-23
Toxicity Effect of Cancer Cell Labeled with Visible Luminescent Nanocrystalline Silicon Particles and Visualization Observation in Vivo Keisuke Sato1, Masaki Hiruoka2, Kohki Fujioka3, Naoki Fukata1, Kenji Hirakuri2, and Kenji Yamamoto3 1 World Premier International (WPI) Research Center, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Scinece (NIMS), 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan 2 Department of Electronic and Computer Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Hikigun, Saitama 350-0394, Japan 3 Department of Medical Ecology and Informatics, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan ABSTRACT Cytotoxicity of human cervical carcinoma cell line (HeLa cells) labeled with the nanocrystalline silicon (nc-Si) particles before and after ultraviolet (UV) light exposure has studied on the viability and cellular membrane damages. The viability and cellular membrane damages of HeLa cells changed at high particle concentration of 1.12 mg/ml. The viability of HeLa cells labeled with the UV-exposed nc-Si particles was higher than that of unexposed nc-Si particles. However, the variation of cellular membrane damages was almost same for the nc-Si particles before and after UV exposure. These results substantiated the low toxicity of nc-Si particles. Moreover, the HeLa cells labeled with the nc-Si particles exhibited green fluorescence. On the other hand, in vivo test of nc-Si particles estimated by the visualization observation of the circulation from the lymphatic vessel to the lymph node of a mouse. The transfer pathway of ncSi particles could be clearly monitored by the strong emission of red light. INTRODUCTION Luminescent semiconductor nanoparticles such as cadmium sulfide (CdS) and cadmium selenide (CdSe) have attracted great attention, because these materials show excellent feature for the optical properties such as size-dependent tunable fluorescence color and high quantum yield of fluorescence [1]. They have been widely studied for various biomedical engineering applications to biological labeling, bio-imaging and diagnostics [2,3]. However, concerns have been raised about the toxicological issue of cadmium in biological systems such as the safety to living organism [4]. That is, the cadmium based materials induce cytotoxicity in vitro. This was correlated with the liberation of free cadmium ions (Cd2+) due to deterioration of CdSe after exposure to ultraviolet (UV) light. Therefore, it is required to develop the new nanoparticle materials consisting of non-toxic and more reliable elements. Nanocrystalline silicon (nc-Si) particles are one of candidate material to eliminate any potential toxicology problems, because they are composed of only harmless elements and have a low environmental impact [5,6]. We have fabricated water-dispersible nc-Si particles by radiofrequency (RF) sputtering and annealing and subsequent treatment in hydrofluoric (HF) acid steam [7]. The nc-Si particles exhibited c
Data Loading...
