Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in color
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(2020) 20:371
BMC Complementary Medicine and Therapies
RESEARCH ARTICLE
Open Access
Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells Hui Zhang1†, Yongbing Wang2†, Jiayang Liu1, Kudelaidi Kuerban1, Jian Li3, Mubarak Iminjan4* and Li Ye1*
Abstract Background: The water extract of Quercuse infectoria galls (QIG) is the active ingredient of Uyghur medicine Xipayi Kui Jie’an (KJA) which has promising therapeutic effects on Ulcerative Colitis (UC) as an alternative medicine. Considering the relationship between UC and the development of colorectal cancer (CRC), the present work aims to explore the direct anti-CRC activity of QIG extract. Methods: CCK8 assay and flow cytometry were used to detect cytotoxicity and apoptosis. Transmission electron microscopy (TEM), flow cytometry, laser confocal and western blotting were performed to examine autophagy. We also adopted Reactive Oxygen Assay kit, as well as transwell and wound healing tests to study the underlying mechanism of QIG against CRC cells. Results: First, we found that QIG extract could suppress the viability of CRC cells and trigger caspases-dependent apoptosis. Subsequently, we proved for the first time that QIG extract also triggered autophagic cell death in CRC cells, which together with apoptosis contributed to the cytotoxic effect on CRC cells. Further investigation revealed that QIG-induced cytotoxicity associated with intracellular ROS accumulation which could suppress the AKT/mTOR signaling pathway, and then induce autophagy and inhibit cell growth. Besides, Erk signaling pathway was also involved in the process of autophagic cell death. Moreover, QIG extract also influenced EMT process and inhibited CRC cell migration. Conclusion: Altogether, this study provides a basis for the utilization of QIG as an alternative medicine for CRC prevention and treatment. Keywords: Quercuse infectoria galls, Colorectal cancer, Apoptosis, Autophagy, Reactive oxygen species
* Correspondence: [email protected]; [email protected] † Hui Zhang and Yongbing Wang contributed equally to this work. 4 Department of pharmaceutical and physical chemistry, College of pharmacy, Xinjiang Medical University, Xinjiang 830011, China 1 Department of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, School of Pharmacy, Fudan University, Shanghai 201203, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the m
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