Transcript profiling combined with functional annotation of 2,662 ESTs provides a molecular picture of Eucalyptus gunnii
- PDF / 770,390 Bytes
- 23 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 146 Views
ORIGINAL PAPER
Transcript profiling combined with functional annotation of 2,662 ESTs provides a molecular picture of Eucalyptus gunnii cold acclimation Guylaine Keller • Phi Bang Cao • Helene San Clemente Walid El Kayal • Christiane Marque • Chantal Teulie`res
•
Received: 22 April 2013 / Revised: 17 July 2013 / Accepted: 26 July 2013 / Published online: 21 August 2013 Springer-Verlag Berlin Heidelberg 2013
Abstract Plants respond to low temperatures through an intricately coordinated transcription network which results in cold acclimation, a phenomenon by which plants increase their freezing tolerance. To analyse the global transcriptome of Eucalyptus gunnii cold-acclimated leaves, 2,662 ESTs were classified by FunCat and the corresponding transcripts quantified throughout a cold acclimation programme using macro-array technique. This combined analysis resulted in the description of the temporal patterns of gene expression with regard to their identity and corresponding functional categories. This paper proposes a predictive hierarchical classification of the main protective mechanisms likely to participate in the
increased cold tolerance of E. gunnii. Given the time course and level of the LEA/dehydrin accumulation, the cryoprotection through proteins may explain most of the hardening. This cryoprotection would be completed by sugar synthesis (raffinose and maltose). As a permanent response, red-ox regulation and protection of membranes or macromolecules against peroxidation look mainly associated with metallothioneins. A limited part of cold response seems to be dedicated to dehydration avoidance through osmoprotectants or to frost avoidance through antifreeze proteins and deposition of cuticle wax. Keywords Eucalyptus Cold acclimation Transcriptional profiling Cryoprotection ROS scavenging
Communicated by F. Canovas. G. Keller, P. B. Cao and H. San Clemente authors contributed equally to this work.
Electronic supplementary material The online version of this article (doi:10.1007/s00468-013-0918-5) contains supplementary material, which is available to authorized users. G. Keller P. B. Cao H. San Clemente W. El Kayal C. Marque C. Teulie`res Universite´ de Toulouse, UPS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326 Castanet-Tolosan, France G. Keller P. B. Cao H. San Clemente W. El Kayal C. Marque C. Teulie`res (&) CNRS, UMR 5546, LRSV, 24 Chemin de Borde Rouge, Auzeville, BP 42617 31326 Castanet-Tolosan, France e-mail: [email protected] Present Address: W. El Kayal Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada
Introduction To survive temperate climates, overwintering plants require the ability to acclimate to freezing temperatures. This cold acclimation, taking place after exposure to chilling temperatures, involves the activation of multiple mechanisms that collectively contribute to transiently enhance freezing tolerance. Many changes at the cellular and molecular levels participate in this complex biolo
Data Loading...
