Molecular Profiling Methods and Protocols
This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery
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Virginia Espina Editor
Molecular Profiling Methods and Protocols Second Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Molecular Profiling Methods and Protocols Second Edition
Edited by
Virginia Espina Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA
Editor Virginia Espina Center for Applied Proteomics and Molecular Medicine George Mason University Manassas, VA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6989-0 ISBN 978-1-4939-6990-6 (eBook) DOI 10.1007/978-1-4939-6990-6 Library of Congress Control Number: 2017937315 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication This book is proudly dedicated to Mary Anne and Len Schiff, for their initial faith in my future scientific career, and to my husband Tito; my children Ben, Paul, and Emily; and my favorite future scientist Olivia, for always listening to my ideas. Virginia Espina
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Preface The first edition of Molecular Profiling (published in 2012) was at the forefront of the personalized medicine movement. The first edition included reviews of genomics and genomic profiling, technologies which in the intervening years have rapidly evolved into routine clinical assays for detecting mutations. Mass spectrometry for protein profiling has also evolved into sensitive and specific multiple reaction monitoring assays, enabling quantification of prot
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