Alternative splicing profiling provides insights into the molecular mechanisms of peanut peg development
- PDF / 3,588,057 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 24 Downloads / 238 Views
RESEARCH ARTICLE
Open Access
Alternative splicing profiling provides insights into the molecular mechanisms of peanut peg development Xiaobo Zhao*†, Chunjuan Li†, Hao Zhang, Caixia Yan, Quanxi Sun, Juan Wang, Cuiling Yuan and Shihua Shan*
Abstract Background: The cultivated peanut (Arachis hypogaea) is one of the most important oilseed crops worldwide, and the generation of pegs and formation of subterranean pods are essential processes in peanut reproductive development. However, little information has been reported about alternative splicing (AS) in peanut peg formation and development. Results: Herein, we presented a comprehensive full-length (FL) transcriptome profiling of AS isoforms during peanut peg and early pod development. We identified 1448, 1102, 832, and 902 specific spliced transcripts in aerial pegs, subterranean pegs, subterranean unswollen pegs, and early swelling pods, respectively. A total of 184 spliced transcripts related to gravity stimulation, light and mechanical response, hormone mediated signaling pathways, and calcium-dependent proteins were identified as possibly involved in peanut peg development. For aerial pegs, spliced transcripts we got were mainly involved in gravity stimulation and cell wall morphogenetic processes. The genes undergoing AS in subterranean peg were possibly involved in gravity stimulation, cell wall morphogenetic processes, and abiotic response. For subterranean unswollen pegs, spliced transcripts were predominantly related to the embryo development and root formation. The genes undergoing splice in early swelling pods were mainly related to ovule development, root hair cells enlargement, root apex division, and seed germination. Conclusion: This study provides evidence that multiple genes are related to gravity stimulation, light and mechanical response, hormone mediated signaling pathways, and calcium-dependent proteins undergoing AS express development-specific spliced isoforms or exhibit an obvious isoform switch during the peanut peg development. AS isoforms in subterranean pegs and pods provides valuable sources to further understand post-transcriptional regulatory mechanisms of AS in the generation of pegs and formation of subterranean pods. Keywords: Arachis hypogaea, Alternative splicing, Full-length transcriptome, Peg development, Gravitropism
Background Peanut (Arachis hypogaea L.) is a widely cultivated oil and cash crop in the world. As one of the members of the Fabaceae family, however, peanut is a unique plant distinct from other legumes, which has the features of the aerial cleistogamous flowers and the subterranean pods [1]. After overground double fertilization, the * Correspondence: [email protected]; [email protected] † Xiaobo Zhao and Chunjuan Li contributed equally to this work. Shandong Peanut Research Institute, Qingdao, China
gynophore develops a specialized geotropic aerial peg by elongation of meristematic cells locating in basal of ovary. The aerial peg grows towards the ground by gravity and penetrates into soil to develop the
Data Loading...
