Transcriptional and post-transcriptional regulation of the miR171- LaSCL6 module during somatic embryogenesis in Larix k
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ORIGINAL ARTICLE
Transcriptional and post‑transcriptional regulation of the miR171‑LaSCL6 module during somatic embryogenesis in Larix kaempferi Qiao‑Lu Zang1 · Yao Zhang2 · Su‑Ying Han2 · Wan‑Feng Li1 · Li‑Wang Qi1 Received: 15 June 2020 / Accepted: 21 August 2020 © The Author(s) 2020
Abstract Key message Expression analysis of Larix kaempferi mature miR171s and their primary transcripts and target gene LaSCL6 during somatic embryogenesis revealed the transcriptional and post-transcriptional regulation of the miR171-LaSCL6 module. Abstract Somatic embryogenesis provides a useful experimental system for studying the regulatory mechanisms of plant development. The level and activity of microRNA171 (miR171) fluctuate during somatic embryogenesis in Larix kaempferi, but the underlying mechanisms are still unclear. Here, in L. kaempferi we identified five members of the miR171 family, which cleave LaSCL6 mRNA at different sites. In addition, we improved the method of measuring miRNA activity in a more direct way. Furthermore, we measured the expression patterns of mature miR171s and their primary transcripts during somatic embryogenesis in L. kaempferi and found that their patterns differed, indicating that the transcription of MIR171 genes and the subsequent cleavage of their intermediate products are regulated. Taken together, our findings not only offer a means to study the regulation of miRNA activity, but also provide further insight into the regulation of L. kaempferi somatic embryogenesis by miR171-LaSCL6. Keywords Gene expression · Japanese larch · LaSCL6 · microRNA171 · microRNA activity · Embryogenic cultures
Introduction Somatic embryogenesis is not only a valuable technique in clonal propagation and genetic improvement, but is also an ideal experimental system in which to study the mechanisms that regulate plant development (Dobrowolska et al. 2017; Godee et al. 2017; Zhang et al. 2019). The induction of embryogenic cultures and the maintenance of embryonic potential are important for clonal plant production through
Communicated by K. Klimaszewska. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00468-020-02026-2) contains supplementary material, which is available to authorized users. * Wan‑Feng Li [email protected] * Li‑Wang Qi [email protected] Extended author information available on the last page of the article
somatic embryogenesis. However, after certain subculture period, the conifer embryogenic cultures may become nonembryogenic and this prevents the formation of somatic embryos and limits utilization of this technique (Klimaszewska et al. 2016; Zhang et al. 2010). Thus, studying the molecular events controlling various stages of somatic embryogenesis could lead to a better understanding of the whole process and potentially improve the efficiency of this technique. Many genetic factors, including microRNAs (miRNAs), have been found to control the process of somatic embryogenesis, such as AGAMOUS-LIKE (Gao et al. 2020), FUSCA (Liu et al. 201
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