Transcriptome analysis of Actinoplanes utahensis reveals molecular signature of saccharide impact on acarbose biosynthes
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ORIGINAL ARTICLE
Transcriptome analysis of Actinoplanes utahensis reveals molecular signature of saccharide impact on acarbose biosynthesis Chun‑Yue Weng1,2,3 · Li‑Zhen Shi1,2,3 · Ya‑Jun Wang1,2,3 · Yu‑Guo Zheng1,2,3 Received: 21 July 2020 / Accepted: 3 October 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Different carbon sources lead to differential acarbose production in Actinoplanes. To uncover the underlying differentiation in the context of genes and pathways, we performed transcriptome sequencing of Actinoplanes utahensis ZJB-03852 grown on different saccharides, such as glucose, maltose, or the saccharide complex consisting of glucose plus maltose. The differentially expressed genes were classified into GO (gene ontology) terms and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways for functional annotations. Key enriched modules were uncovered. Our data revealed that both maltose and its complex with glucose gave improved acarbose titer. Sugar transportation, cytochrome oxidase, protein synthesis and amino acid metabolism modules were enriched under the saccharide complex condition, while ferritin metabolism gene expressions were enriched in the glucose medium. Our results provided the foundation for uncovering the mechanism of carbon source on acarbose production in A. utahensis. Keywords Acarbose · Actinoplanes utahensis · Transcriptome · Saccharide Abbreviations ACN Acetonitrile CDW Cell dry weight DEG Differentially expressed genes FPKM Fragments per kilobase per million mapped reads Glc A. utahensis ZJB-03852 cultured with 80 g/L glucose as the carbon source GM A. utahensis ZJB-03852 cultured with 40 g/L glucose and 40 g/L maltose as the carbon source Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02466-0) contains supplementary material, which is available to authorized users. * Ya‑Jun Wang [email protected] 1
The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014, People’s Republic of China
2
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, People’s Republic of China
3
Engineering Research Center of Bioconversion and Biopurification of the Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, People’s Republic of China
GO Gene ontology KEGG Kyoto Encyclopedia of Genes and Genomes Mal A. utahensis ZJB-03852 cultured with 80 g/L maltose as the carbon source NRPS Non-ribosomal peptide synthetase PKS Polyketide synthase PPI Protein–protein interaction T2DM Type 2 diabetes mellitus
Introduction Diabetes mellitus is a worldwide metabolic disease characterized by chronic hyperglycemia (American Diabetes Association 2019), which severely impairs the patients’ life quality. In 2019, 463 million adults (20–79 years) were diagnosed with d
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