Transcriptome analysis of Eucalyptus grandis genotypes reveals constitutive overexpression of genes related to rust ( Au
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Transcriptome analysis of Eucalyptus grandis genotypes reveals constitutive overexpression of genes related to rust (Austropuccinia psidii) resistance Samuel A. Santos1,4 · Pedro M. P. Vidigal2 · Lúcio M. S. Guimarães1 · Reginaldo G. Mafia3 · Matthew D. Templeton4 · Acelino C. Alfenas1,5 Received: 15 November 2019 / Accepted: 29 June 2020 © Springer Nature B.V. 2020
Abstract Key Message A resistant E. grandis genotype showed a constitutive overexpression of genes related to resistance to myrtle rust caused by A. psidii. Abstract Myrtle rust caused by Austropuccinia psidii is considered one of the most important fungal diseases affecting Eucalyptus spp. plantations in Brazil. Although the selection and planting of resistant eucalypt genotypes have been the major strategies to manage the disease in Brazil, the molecular mechanisms involved in resistance are still unclear. In this study, we evaluated the gene expression profile of two contrasting Eucalyptus grandis genotypes in resistance level to rust by RNA-Seq. The two genotypes showed a very different background gene expression level even without A. psidii infection. The resistant genotype had a constitutive overexpression of a large number of protein-coding genes compared to the susceptible genotype. These genes were mainly associated with signal transduction, photosynthesis, regulation and response to salicylic acid (SA), and protein kinase leucine-rich receptors (PK-LRR). PK-LRR and SA mediated disease resistance are well known to be effective against obligate biotroph pathogens, such as A. psidii. In addition, at 24 h after infection, the susceptible genotype was able to activate some response, however, several resistance-related proteins had their expression level reduced with A. psidii infection. Here, we present the first analysis of E. grandis genotypes transcriptomes infected by A. psidii and it reveals a constitutive overexpression of several resistance-related genes in the resistant genotype compared to the susceptible one. Our findings have the potential to be used as candidate molecular markers for resistance to myrtle rust. Keywords Myrtle rust · RNA-Seq · DEGs · Protein kinase · Leucine-rich receptors Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01030-x) contains supplementary material, which is available to authorized users. * Acelino C. Alfenas [email protected] 1
Laboratory of Forest Pathology, Department of Plant Pathology, Universidade Federal de Viçosa, Viçosa, MG 36570‑900, Brazil
2
Núcleo de Análise de Biomoléculas (NuBioMol), Centro de Ciências Biológicas, Universidade Federal de Viçosa, Viçosa, MG 36570‑900, Brazil
3
Centro de Tecnologia, Suzano S.A., Aracruz, ES, Brazil
4
The New Zealand Institute for Plant and Food Research Limited, Auckland 1142, New Zealand
5
Department of Plant Pathology, Instituto de Biotecnologia Aplicada à agropecuária‑BIOAGRO, Universidade Federal de Viçosa, Av. P.H. Rolfs s/n, Campus Universitário, Viçosa, MG 36570‑900, Bra
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