Transcriptome analysis reveals modulation of the STAT family in PEDV-infected IPEC-J2 cells
- PDF / 2,309,674 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 109 Downloads / 174 Views
RESEARCH ARTICLE
Open Access
Transcriptome analysis reveals modulation of the STAT family in PEDV-infected IPEC-J2 cells Zhengzheng Hu1, Yuchen Li1, Heng Du1, Junxiao Ren1, Xianrui Zheng1, Kejian Wei2 and Jianfeng Liu1*
Abstract Background: Porcine epidemic diarrhea virus (PEDV) is a causative agent of serious viral enteric disease in suckling pigs. Such diseases cause considerable economic losses in the global swine industry. Enhancing our knowledge of PEDV-induced transcriptomic responses in host cells is imperative to understanding the molecular mechanisms involved in the immune response. Here, we analyzed the transcriptomic profile of intestinal porcine epithelial cell line J2 (IPEC-J2) after infection with a classical strain of PEDV to explore the host response. Results: In total, 854 genes were significantly differentially expressed after PEDV infection, including 716 upregulated and 138 downregulated genes. Functional annotation analysis revealed that the differentially expressed genes were mainly enriched in the influenza A, TNF signaling, inflammatory response, cytokine receptor interaction, and other immune-related pathways. Next, the putative promoter regions of the 854 differentially expressed genes were examined for the presence of transcription factor binding sites using the MEME tool. As a result, 504 sequences (59.02%) were identified as possessing at least one binding site of signal transducer and activator of transcription (STAT), and five STAT transcription factors were significantly induced by PEDV infection. Furthermore, we revealed the regulatory network induced by STAT members in the process of PEDV infection. Conclusion: Our transcriptomic analysis described the host genetic response to PEDV infection in detail in IPEC-J2 cells, and suggested that STAT transcription factors may serve as key regulators in the response to PEDV infection. These results further our understanding of the pathogenesis of PEDV. Keywords: PEDV, IPEC-J2, RNA-seq, STAT family
Background Porcine epidemic diarrhea (PED), caused by the porcine epidemic diarrhea virus (PEDV), is one of the most severe and globally widespread infectious diseases affecting swine of all ages. PED has resulted in significant economic losses to the pig industry over the past three decades. It was first observed in Europe in 1971 [1]. Outbreaks occurred in Asian countries in 1982 and since then, PED has had a * Correspondence: [email protected] 1 National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China Full list of author information is available at the end of the article
growing economic impact on the Asian swine industry [2]. In 2013, PEDV was first reported in the US and spread rapidly nationwide [3]. PED is characterized by severe diarrhea, vomiting, dehydration, and a mortality rate of up to 90% in suckling piglets [4]. Domestic pig farms in China have been using the epidemic diarrhea inactivated vac
Data Loading...
