Transcriptome analysis reveals metabolic regulation mechanism of microalga Chlorella pyrenoidosa in response to the mixe
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Transcriptome analysis reveals metabolic regulation mechanism of microalga Chlorella pyrenoidosa in response to the mixed culture with yeast Yarrowia lipolytica Lei Qin 1,2,3,4 & Dong Wei 4 Received: 20 December 2019 / Revised and accepted: 15 April 2020 # Springer Nature B.V. 2020
Abstract The mixed culture of microalgae and yeast has been confirmed to enhance many bioprocesses including waste degradation, remediation, and biomass generation for lipid production. Our latest study showed that besides the higher yield of biomass and lipid, the mixed culture of Chlorella pyrenoidosa and Yarrowia lipolytica can achieve the higher yields of carbohydrates, protein, and higher heating value. The strengthening mechanism of the mixed culture of microalgae and yeast mostly focused on nutritional complementarity and the culture conditions in previous works. In this work, de novo transcriptome profiling and central metabolic pathway analysis of C. pyrenoidosa cells in mono microalgae culture and mixed culture were performed. The differential transcriptome analysis revealed that most biological processes of C. pyrenoidosa, such as TCA cycle, glycolysis/ gluconeogenesis pathway, pentose phosphate pathway, and nitrate assimilation, were enhanced by the mixed culture with yeast at 24 h. Meanwhile, the CO2 assimilation of C. pyrenoidosa was inhibited. Deep analysis of carbon metabolism, nitrogen metabolism, and photosynthesis well illustrates the intrinsic mechanism of physiological and biochemical differences occurring in the mono microalgae culture and the mixed culture. This study unraveled metabolic regulation molecular mechanism of C. pyrenoidosa in response to the mixed culture with Y. lipolytica, and strengthens the understanding of the synergistic effect between microalgae and yeast in the mixed culture. Keywords Chlorella pyrenoidosa . Yarrowia lipolytica . Mixed culture . Transcriptome . De novo analyses
Introduction Mixed culture of microorganisms is common in natural ecological systems. It is often used for the treatment of waste materials discharged from industries, as well as for the production of biomass and bioactive compounds (Cheirsilp et al. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10811-020-02135-y) contains supplementary material, which is available to authorized users. * Dong Wei [email protected] 1
Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China
2
CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China
3
Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China
4
School of Food Science and Engineering, South China University of Technology, Wushan Rd. 381, Guangzhou 510641, China
2011). In recent years the mixed culture of photosynthetic microalgae and non-photosynthetic yeast has attracted wide attention (Qin et al. 2017). The mixed culture of microalgae and yeast could be a strategy to improve wastewater and/or low-cost carbon source
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