Transcriptome Analysis Reveals Potential Genes Involved in Digestive Enzyme Function in a Mudflat Crab Helice tientsinen
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Transcriptome Analysis Reveals Potential Genes Involved in Digestive Enzyme Function in a Mudflat Crab Helice tientsinensis Zhengfei Wang 1
&
Dan Tang 1 & Huayun Guo 1 & Xueling Shi 1
Received: 4 December 2019 / Revised: 21 February 2020 / # Springer Nature Switzerland AG 2020
Abstract The mudflat crab (Helice tientsinensis) is one of the most common crabs along the coast of China, and it is one of the important commercial species in Chinese aquaculture. However, unlike other consumed crabs, which are omnivores or carnivores, H. tientsinensis is a primarily herbivorous crab. Studies have rarely been conducted on the expression of digestive enzyme genes and their role in regulating digestive capacity in this crab. Here, we applied the Illumina platform to sequence and de novo assembled a H. tientsinensis transcriptome dataset using mRNA from the hepatopancreas. A total of 50,730,758 clean reads were obtained and 399,653 unigenes were assembled. Among these unigenes, 36,221 unigenes had signifificant hits in the NR protein database. These unigenes were predicted to encode 127 carbohydrases, 107 peptidases and 9 lipases. We observed that the glucanases and amylases were most closely related to those found in other Decapoda species. RT-qPCR analysis showed that the expression of 6 digestive enzyme genes was higher in the hepatopancreas than those in other parts of the body. It is the first time to report various digestive enzyme genes in H. tientsinensis and to provide a better understanding about the physiological adaptation to primarily herbivorous diet. Keywords Transcriptome . Hepatopancreas . Digestive enzyme . Crabs . Helice tientsinensis
Introduction Digestive enzyme activity is the most common indicator for evaluating the capacity of digestion (Dai et al. 2009). The digestion of food to obtain nutrients is a physiological function of digestive enzymes (Wei et al. 2014), and the digestive enzymes of animals with different feeding habits (carnivores, omnivores and herbivores) have different characteristics (Wang et al. 2016). In crustaceans, the hepatopancreas is an important organ for the absorption and storage of nutrients, which can synthesize digestive enzymes for food digestion.
Electronic Supplementary Material The online version of this article (https://doi.org/10.1007/s41208-020-00212-0) contains supplementary material, which is available to authorized users. * Zhengfei Wang [email protected] 1
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng 224001, Jiangsu Province, People’s Republic of China
Studies on hepatopancreatic secretions have identified trypsin, chymotrypsin, amylase and lipase as the most important components for digestion in crustacean species (Fernández et al. 1997; Hammer et al. 2000; Wei et al. 2014). However, though the digestive enzyme activity and nutrit
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