Transcriptome sequencing and gene expression profiling of Populus wutunensis , a natural hybrid, during salinity stress
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ORIGINAL ARTICLE
Transcriptome sequencing and gene expression profiling of Populus wutunensis, a natural hybrid, during salinity stress Jixiang Zou1,2 · Hua Jin1,2 Received: 29 October 2019 / Accepted: 17 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message When Populus wutunensis seedlings are salt stressed, many genes involved in cellular metabolism, signal transduction, transport, osmotic regulation, and reactive oxygen species scavenging show elevated expression levels. Abstract Populus wutunensis, a natural hybrid individual resulting from a cross between Populus × xiaozhuanica W. Y. Hsu et Liang, shows salt and drought tolerance and is fast-growing. It is widely cultivated in the Three-North Shelterbelt Forest area in China. To determine the transcriptome changes in P. wutunensis under salt stress, RNA-seq was conducted in salt-treated leaves and non-salt-treated control leaves. A total of 81,188 unigenes, with an average length of 982 bp, were obtained. In salt-treated leaves, 2466 differentially expressed genes were identified that differed from those in control leaves at any time point. Among them, 391 were upregulated and 44 were downregulated during all salt stress time points. They were mainly involved in cellular and metabolic processes, signal transduction, transport, osmotic regulation, and reactive oxygen species (ROS) scavenging. In addition, 2543 transcription factor genes were identified and divided into 60 families. qRT-PCR analysis revealed that the levels of differentially expressed genes were consistent with those from sequencing of the transcriptome under salt stress. These findings will increase our understanding of gene regulation in response to salt stress in P. wutunensis and are beneficial for future research on molecular-assisted breeding. Keywords Differentially expressed gene · Populus wutunensis · Salt stress · Transcription factor Abbreviations ROS Reactive oxygen species qRT-PCR Quantitative reverse transcription polymerase chain reaction DEGs Differentially expressed genes
Communicated by B. Wang. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00468-020-02014-6) contains supplementary material, which is available to authorized users. * Hua Jin [email protected] 1
College of Environment and Resource, Dalian Nationalities University, Liaoning University, Dalian 116600, China
Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Institute of Plant Resources, Dalian Minzu University, Dalian 116600, China
2
Introduction Soil salinization has become an important challenge in managing global resources and conserving the environment as it reduces productivity (Nawar et al. 2015; Zhu et al. 2016). Areas supporting major crops, affected by salinity, alkalinity, and extreme environments worldwide, are > 50% annually. If saline-alkali lands can be improved and utilized, they can provide significantly more ecological and economic value to society. The
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