Transcriptome sequencing and metabolite profiling analyses provide comprehensive insight into molecular mechanisms of fl
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Transcriptome sequencing and metabolite profiling analyses provide comprehensive insight into molecular mechanisms of flower development in Dendrobium officinale (Orchidaceae) Chunmei He1 · Xuncheng Liu1 · Jaime A. Teixeira da Silva2 · Nan Liu3 · Mingze Zhang1,4 · Jun Duan1,5 Received: 30 April 2020 / Accepted: 18 August 2020 © Springer Nature B.V. 2020
Abstract Key message This research provides comprehensive insight into the molecular networks and molecular mechanisms underlying D. officinale flower development. Abstract Flowers are complex reproductive organs and play a crucial role in plant propagation, while also providing sustenance for insects and natural bioactive metabolites for humans. However, knowledge about gene regulation and floral metabolomes in flowers is limited. In this study, we used an important orchid species (Dendrobium officinale), whose flowers can be used to make herbal tea, to perform transcriptome sequencing and metabolic profiling of early- and medium-stage flower buds, as well as opened flowers, to provide comprehensive insight into the molecular mechanisms underlying flower development. A total of 8019 differentially expressed genes (DEGs) and 239 differentiated metabolites were found. The transcription factors that were identified and analyzed belong exclusively to the MIKC-type MADS-box proteins and auxin responsive factors that are known to be involved in flower development. The expression of genes involved in chlorophyll and carotenoid biosynthesis strongly matched the metabolite accumulation patterns. The genes related to flavonoid and polysaccharide biosynthesis were active during flower development. Interestingly, indole-3-acetic acid and abscisic acid, whose trend of accumulation was inverse during flower development, may play an important role in this process. Collectively, the identification of DEGs and differentiated metabolites could help to illustrate the regulatory networks and molecular mechanisms important for flower development in this orchid. Keywords Differential expression genes · Pigment biosynthesis · Polysaccharide biosynthesis · Plant hormones · RNAsequence · Metabolomics
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01058-z) contains supplementary material, which is available to authorized users. * Jun Duan [email protected] Chunmei He [email protected] Xuncheng Liu [email protected] Jaime A. Teixeira da Silva [email protected] Nan Liu [email protected] Mingze Zhang [email protected]
1
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2
Kagawa‑ken, Japan
3
Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
4
University of the Chinese Academy of Sciences, Beijing 100049, China
5
Center of Economic Botany, Core Botanical G
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