Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW26
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Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW264.7 macrophage cells ZHANG Pingping1, LI Jiang2*, YU Leiye3, 4, WEI Jingfang1, XU Tong1, SUN Guojie1* 1 College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018,
China 2 Key Laboratory of Marine Bioactive Substances, the First Institute of Oceanography, State Oceanic Administration,
Qingdao 266061, China 3 Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 4 University of Chinese Academy of Sciences, Beijing 100049, China
Received 1 December 2017; accepted 12 April 2018 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract
B-3 exopolysaccharide is extracted from the Antarctic psychrophilic bacterium Psychrobacter sp. B-3. We have previously shown that it activates macrophages and affects their immunoregulatory activities. To determine what genes are affected during this process, we detected the genes differentially expressed in cells of RAW264.7 macrophages treated with B-3 exopolysaccharide by transcriptomic analysis. B-3 exopolysaccharide treatment caused differential expression of 420 genes, of which 178 were up-regulated and 242 were down-regulated. These genes were shown to be involved in many aspects of cell function, mainly metabolism and immunity. Genes were enriched in multiple immune-related pathways, and the most significantly enriched genes were involved in antigen processing and presentation pathways. The pathway in which differentially expressed genes were the most significantly enriched was the metabolic pathway; specifically, the expression of many metabolic enzyme genes was altered by B-3 exopolysaccharide treatment. Additionally, the genes involved in metabolisms of amino acids, carbohydrates, lipids and nucleotides, varied to certain degrees. B-3 exopolysaccharide, therefore, appears to directly affect the immune function of RAW264.7 macrophages as an immunostimulant, or to indirectly change intracellular metabolism. This is the first study to determine the effect of an Antarctic psychrophilic bacterial exopolysaccharide on RAW264.7 macrophages. Our findings provide an important reference for research into the regulation of macrophage immune function by different polysaccharides. Key words: Psychrobacter sp. B-3, B-3 exopolysaccharide, RAW264.7 macrophage, differentially expressed genes, immunological pathway, metabolic pathway Citation: Zhang Pingping, Li Jiang, Yu Leiye, Wei Jingfang, Xu Tong, Sun Guojie. 2018. Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW264.7 macrophage cells. Acta Oceanologica Sinica, 37(8): 46–53, doi: 10.1007/s13131-018-1227-z
1 Introduction Polysaccharides from fungi, plants, bacteria, and animals have been shown to exhibit a variety of biological activities, including antitumor (Wasser, 2002; Lemieszek and Rzeski, 2012), immunoregulatory (Wakabaya
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