Transcriptome analysis of the Antarctic psychrotrophic bacterium Psychrobacter sp. G in response to temperature stress
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Transcriptome analysis of the Antarctic psychrotrophic bacterium Psychrobacter sp. G in response to temperature stress WANG Zhen1, LI Yang1, LIN Xuezheng1* 1 The First Institute of Oceanography, Key Laboratory of Marine Bioactive Substances, State Oceanic Administration,
Qingdao 266061, China Received 31 August 2015; accepted 15 May 2016 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2017
Abstract
The key functional genes involved in temperature adaption of the Antarctic psychrotrophic bacterium Psychrobacter sp. G. were identified by transcriptomic sequencing. We analyzed the global transcriptional profile of Psychrobacter sp. G under cold stress (0°C) and heat stress (30°C), with the optimal growth temperature 20°C as the control. There were large alterations of the transcriptome profile, including significant upregulation of 11 and 12 transcripts as well as significant downregulation of 47 and 42 transcripts in the cold and heat stress groups, respectively, compared to the control. The expression of various genes encoding enzymes and transcriptional regulators, including PfpI and TetR family transcriptional regulators under heat stress, as well as the expression of DEAD/DEAH box helicase and the IclR family of transcriptional regulators under cold stress, were upregulated significantly. The expression of several genes, most affiliated with TonBdependent receptor and siderophore receptor, was downregulated significantly under both heat and cold stress. Many of the genes associated with the metabolism of fatty acid and ABC transporters were regulated differentially under different temperature stress. The results of this survey of transcriptome and temperature stress-relevant genes contribute to our understanding of the stress-resistant mechanism in Antarctic bacteria. Key words: Psychrobacter, temperature stress, transcriptome, Antarctic Citation: Wang Zhen, Li Yang, Lin Xuezheng. 2017. Transcriptome analysis of the Antarctic psychrotrophic bacterium Psychrobacter sp. G in response to temperature stress. Acta Oceanologica Sinica, 36(2): 78–87, doi: 10.1007/s13131-016-0956-0
1 Introduction Characteristics of the Antarctic ecosystem include temperatures below freezing for long periods, severe aridity, limited availability of nutrients and intense UV radiation (Pearce, 2008; Turner et al., 2005). Many microorganisms have important roles in the material circulation of the Antarctic ecosystem (Zeng and Chen, 1999). Cold-adapted microorganisms have colonized cold environments from mountains to deep sea and polar regions (Suresh et al., 2011). To survive in this severe environment, bacteria have developed complex mechanisms to cope with environmental stress (Ferenci and Spira, 2007). Consequently, Antarctica is an excellent place for evolutionary studies under extreme environmental conditions, which is helpful for genetic engineering studies (Feller and Gerday, 2003). Characterization of a transcriptome, which is the repertoire of expressed RNA transcripts in a cell, is essential for decryp
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