Transcriptomic and anatomic profiling reveal the germination process of different wheat varieties in response to waterlo
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RESEARCH ARTICLE
Open Access
Transcriptomic and anatomic profiling reveal the germination process of different wheat varieties in response to waterlogging stress Changwei Shen1, Jingping Yuan2, Hong Qiao3, Zijuan Wang3, Yuanhai Liu3, Xiujuan Ren1, Fei Wang1, Xing Liu1, Ying Zhang1, Xiling Chen1 and Xingqi Ou4*
Abstract Background: Waterlogging is one of the most serious abiotic stresses affecting wheat-growing regions in China. Considerable differences in waterlogging tolerance have been found among different wheat varieties, and the mechanisms governing the waterlogging tolerance of wheat seeds during germination have not been elucidated. Results: The results showed no significant difference between the germination rate of ‘Bainong 207’ (BN207) (after 72 h of waterlogging treatment) and that of the control seeds. However, the degree of emulsification and the degradation rate of endosperm cells under waterlogging stress were higher than those obtained with the control treatment, and the number of amyloplasts in the endosperm was significantly reduced by waterlogging. Transcriptomic data were obtained from seed samples (a total of 18 samples) of three wheat varieties, ‘Zhoumai 22’ (ZM22), BN207 and ‘Bainong 607’ (BN607), subjected to the waterlogging and control treatments. A comprehensive analysis identified a total of 2775 differentially expressed genes (DEGs). In addition, an analysis of the correlations among the expression difference levels of DEGs and the seed germination rates of the three wheat varieties under waterlogging stress revealed that the relative expression levels of 563 and 398 genes were positively and negatively correlated with the germination rate of the wheat seeds, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the difference in the waterlogging tolerance among the three wheat varieties was related to the abundance of key genes involved in the glycolysis pathway, the starch and sucrose metabolism pathway, and the lactose metabolism pathway. The alcohol dehydrogenase (ADH) gene in the endosperm of BN607 was induced immediately after short-term waterlogging, and the energy provided by the glycolysis pathway enabled the BN607 seeds to germinate as early as possible; in addition, the expression of the AP2/ERF transcription factor was upregulated to further enhance the waterlogging tolerance of this cultivar. Conclusions: Taken together, the results of this study help elucidate the mechanisms through which different wheat varieties respond to waterlogging stress during germination. Keywords: Waterlogged, Wheat, Endosperm, Anatomical structure, Transcriptome, Differentially expressed gene
* Correspondence: [email protected] 4 School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use,
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