Transcriptomic and proteomic profiling response of methicillin-resistant Staphylococcus aureus (MRSA) to a novel bacteri
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GENOMICS, TRANSCRIPTOMICS, PROTEOMICS
Transcriptomic and proteomic profiling response of methicillin-resistant Staphylococcus aureus (MRSA) to a novel bacteriocin, plantaricin GZ1-27 and its inhibition of biofilm formation Hechao Du 1 & Libang Zhou 1 & Zhaoxin Lu 1
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Xiaomei Bie 1
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Haizhen Zhao 1
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Yan D. Niu 2 & Fengxia Lu 1
Received: 21 November 2019 / Revised: 9 March 2020 / Accepted: 25 March 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug, due to its wide distribution and multidrug resistance. To characterize effects of a newly identified plantaricin GZ1-27 on MRSA, transcriptomic and proteomic profiling of MRSA strain ATCC43300 was performed in response to sub-MIC (16 μg/mL) plantaricin GZ1-27 stress. In total, 1090 differentially expressed genes (padj < 0.05) and 418 differentially expressed proteins (fold change > 1.2, p < 0.05) were identified. Centralized protein expression clusters were predicted in biological functions (biofilm formation, DNA replication and repair, and heat-shock) and metabolic pathways (purine metabolism, amino acid metabolism, and biosynthesis of secondary metabolites). Moreover, a capacity of inhibition MRSA biofilm formation and killing biofilm cells were verified using crystal violet staining, scanning electron microscopy, and confocal laser-scanning microscopy. These findings yielded comprehensive new data regarding responses induced by plantaricin and could inform evidence-based methods to mitigate MRSA biofilm formation. Keywords MRSA . Plantaricin . Transcriptomic . Proteomic . Biofilm
Introduction Methicillin-resistant Staphylococcus aureus (MRSA) is considered a global pandemic threat (Rossolini et al. 2014). As reported, the MRSA incidence exceeds 20% in all 85 investigated World Health Organization (WHO) member states (Álvarez et al. 2019). Since it was first reported in 1961 (Jevons 1961), MRSA has thrived in hospitals, communities, and livestock worldwide (Wendlandt et al. 2013). In the past decade, various MRSA genotypes were identified in raw meat (pork, poultry, and beef) and milk, indicating a high risk of
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10589-w) contains supplementary material, which is available to authorized users. * Fengxia Lu [email protected] 1
College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China
2
Faculty of Veterinary Medicine, University of Calgary, Calgary T2N 4Z6, Canada
MRSA in food-borne transmission (Titouche et al. 2019). As one of the most common antibiotic-resistant bacteria, MRSA were resistant to most β-lactam antibiotics, including methicillin, penicillin, carbapenems, cephalosporins, and their derivatives (Doyle et al. 2012). A biofilm is a community of organisms encased in a protective and adhesive matrix. Biofilms allow MRSA strains to adhere to various biotic and abiotic surfaces, including c
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