Traumatic and Ischemic Injury Methods and Protocols

This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumat

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Binu Tharakan Editor

Traumatic and Ischemic Injury Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Traumatic and Ischemic Injury Methods and Protocols

Edited by

Binu Tharakan Department of Surgery, Texas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research Institute, Temple, TX, USA

Editor Binu Tharakan Department of Surgery Texas A&M University Health Science Center College of Medicine, Baylor Scott and White Research Institute Temple, TX, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7524-2 ISBN 978-1-4939-7526-6 (eBook) https://doi.org/10.1007/978-1-4939-7526-6 Library of Congress Control Number: 2018931384 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Endothelial cells of the blood-brain barrier stained for zonula occludents-1 (green) and nucleus (blue). Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Traumatic and ischemic injuries are some of the most devastating diseases and are responsible for high mortality and morbidity worldwide. Over the past decades, significant research has been conducted in this area, aimed at reducing or preventing complications after a traumatic or ischemic injury. Although some advances have been made in our understanding of the pathobiology of such insults, further research and innovative approaches are essential to clearly understand and treat these complex issues. The use of a suit