Trephine- and dye-free technique for eye bank preparation of pre-stripped Descemet membrane endothelial keratoplasty tis
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Trephine- and dye-free technique for eye bank preparation of pre-stripped Descemet membrane endothelial keratoplasty tissue Tahereh Chamani . Mohammad Ali Javadi . Mozhgan Rezaei Kanavi
Received: 8 September 2018 / Accepted: 10 May 2019 / Published online: 11 May 2019 Ó Springer Nature B.V. 2019
Abstract We describe a rapid and easy technique for the preparation of pre-stripped Descemet membrane (DM) endothelial keratoplasty (DMEK) tissue in the eye bank, without the use of dye or trephining by the eye bank technician. After drawing 2 radial marks 90° apart on the scleral rim of the cornea, a circumferential incision was made internal to the trabecular meshwork and the edge of the DM opposite to the marked area was scored and gently peeled towards the marked area. Finally, the peeled DM was gently unrolled and attached to the posterior stromal bed via absorbing excess liquid from the periphery. The prepared DMEK tissue while reattached on the posterior aspect of the cornea was subjected to slit-lamp biomicroscopic and specular microscopic examinations. In case of proper preparation of DMEK tissue and suitability of endothelial cells for DMEK surgery, the tissue was distributed for transplantation. At the time of surgery, trypan blue staining was performed by the cornea
T. Chamani Central Eye Bank of Iran, Tehran, Iran M. A. Javadi Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran M. R. Kanavi (&) Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran e-mail: [email protected]; [email protected]
surgeon before DM trephination. Duration of DMEK tissue preparation, uneventful preparation, post-preparation endothelial cell loss, surgeon’s satisfaction, and intra- and post-operative attachment of the tissue were investigated variables in this study. Over a 2-year period, 28 DMEK tissues were successfully prepared from 30 corneas using our new technique. The median duration of DMEK preparation was 100 s. The area of endothelial cell loss was 1.8% ± 1.2% centrally and 6.2% ± 1.8% in areas close to the forceps contacts. Seven out of the 28 (25%) DMEK tissues were ruined by surgeons during surgery and the overall rate of rebubbling in 19 transplanted DMEK tissues was 16%. The presented method, which uses no trephining or vital dye, has simplified and expedited the preparation process of pre-stripped DMEK tissue in the eye banks. Keywords Descemet membrane endothelial keratoplasty DMEK Re-bubbling
Introduction Descemet membrane endothelial keratoplasty (DMEK) with selective replacement of impaired endothelial cells confers faster visual recovery and a smaller graft rejection rate than Descemet stripping automated endothelial keratoplasty (DSAEK) (Boynton and Woodward 2014; Tourtas et al. 2012; Guerra
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et al. 2011; Anshu et al. 2012). However, challenges with donor preparation, handling of the delicate tissue, and surgical learning curve have limited the use of DMEK by
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