Tryptophan decreases the intensity of lipopolysaccharide-induced acute lung injury in a rat model
- PDF / 1,640,002 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 22 Downloads / 160 Views
ORIGINAL ARTICLE
Tryptophan decreases the intensity of lipopolysaccharide‑induced acute lung injury in a rat model Shuangqing Liu1,2,3 · Fei She2 · Wei Zhang2 · Xia Hu2 · Xiaodong Zhao2 · Yongming Yao1,3 Received: 21 March 2020 / Accepted: 26 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Sepsis is a severe clinical condition that is a result of the cellular and biochemical response to infection. The present study evaluated the therapeutic potential of tryptophan against lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. Rats were grouped into sham, control (ALI), and ALI + 1, 25, and 50 mg/kg body weight l-tryptophan. Supplementation with 1, 25, and 50 mg/kg l-tryptophan reduced the total protein content by 4.9%, 33.4%, and 64.5%; the levels of neutrophils (12.5%, 31.8%, and 65.1%), lymphocytes (15.1%, 41.7%, and 63.3%), total cells (12.6%, 42.4%, and 65.7%); lipid peroxidation (9.4%, 28.4%, and 68.7%); myeloperoxidase levels (12.1%, 33.4%, and 68.2%); migration inhibitory factor (12.7%, 39.5%, and 68.2%), interleukin (IL)-8 (5.5%, 46.8%, and 78.5%), tumor necrosis factor (TNF)-α (10.8%, 39.8%, and 72.2%), respectively. Supplementation with 1, 25, and 50 mg/kg l-tryptophan reduced mRNA expression of TNF-α (4.5%, 21.8%, and 41.8%), IL-1β (5.2%, 17.9%, and 46.2%); and the protein expression of TNF-α (2.8%, 15.2%, and 35.7%) and IL-1β (5.2%, 15.6%, and 28.6%), respectively. It also reduced glutathione (to near normal levels), neutrophilic infiltration and edema, and the wet/dry ratio of lung tissue. It significantly increased catalase, superoxide dismutase, glutathione peroxidase levels, as well as the partial pressure of oxygen (PaO2) by 21.9%, 52.8%, and 87.4%, respectively. Altogether, our results suggest that supplementation with l-tryptophan has a strong protective effect against LPS-induced ALI. Keywords l-Tryptophan · Sepsis · Acute lung injury · Rats · Antioxidant Abbreviations ALI Acute lung injury MPO Myeloperoxidase TNF-α Tumor necrosis factor alpha MIF Migration inhibitory factor IL-8 Interleukin-8 PaO2 Partial pressure of oxygen ARDS Acute respiratory distress syndrome BALF Bronchoalveolar lavage fluid MDA Malondialdehyde ANOVA Analysis of variance
Handling editor: J. Broos. * Yongming Yao [email protected] 1
Medical school of Chinese PLA, Beijing, China
2
Department of Emergency, the Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China
3
Trauma Research Center, the Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China
GSH Reduced glutathione SOD Superoxide dismutase
Introduction Sepsis is a severe clinical condition that is a result of the cellular and biochemical response to infection (Zhai et al. 2018). Baracchi et al. (2011) reported a higher rate of sepsis-induced mortality in individuals in intensive care. Sepsis causes dysfunction in lungs and other organ (Kim and Hong 2016). In particular, patients with tachypnea and hypoxia experience acute lung injury (ALI) (Raghavend
Data Loading...
