The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation
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ORIGINAL ARTICLE
The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation Zhiqiang Ye 1 & Xuhui Liu 1 & Yuewu Yang 2 & Xianling Zhang 3 & Ting Yu 1 & Shigeng Li 1 & Yawei Feng 4 & Gangjian Luo 4
Received: 1 August 2017 / Accepted: 6 November 2017 # University of Navarra 2017
Abstract Acute lung injury caused by smoke inhalation is a common severe clinical syndrome. This study aimed to investigate the potential expression of circular RNAs during acute lung injury triggered by smoke inhalation. The acute lung injury rat model was established with smoke inhalation from a self-made smoke generator. The occurrence of acute lung injury was validated by an analysis of the bronchoalveolar lavage fluid and hematoxylin-eosin (HE) staining of lung tissues. Next-generation sequencing and quantitative PCR were performed to identify the differentially expressed circular RNAs associated with acute lung injury that was caused by smoke inhalation. The circular form of the identified RNAs was finally verified by multiple RT-PCR-based assays. The bronchoalveolar lavage fluid (BALF) and lung tissue analysis showed that smoke inhalation successfully induced acute injury in rats, as evidenced by the significantly altered cell numbers, including macrophages, neutrophils, and red blood cells, disrupted cell lining, and increased levels of interleukin-1β, tumor necrosis factor-alpha, and IL-8 in lung tissues. Ten significantly differentially expressed circular RNAs were
Zhiqiang Ye and Xuhui Liu contributed equally to this work. * Gangjian Luo [email protected] 1
Department of Emergency, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
2
Department of Traditional Chinese Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
3
Department of Hepatic Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
4
Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, No. 600, tianhe road, tianhe district, Guangzhou 510000, China
identified with next-generation sequencing and RT-PCR. The circular form of these RNAs was verified by multiple RT-PCR-based assays. In conclusion, the identified circular RNAs were prevalently and differentially expressed in rat lungs after acute lung injury caused by smoke inhalation. Keywords Acute lung injury . Smoke inhalation . Circular RNA . Rat model
Introduction Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) refer to the common acute respiratory failure associated with substantial morbidity and mortality worldwide. They usually feature lung endothelial and epithelial barrier disruption and could lead to impaired long-term quality of life, even for those who survive ALI [18, 20]. Patients with ALI commonly suffer from disrupted alveolar–capillary membrane integrity. This membrane is composed of the microvascular endothelium, interstitium, and alveolar epithelium, and its disruption consists of the release of cytotoxic a
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