Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration
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Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration Lı´via Contini Massimino . Virginia da Conceic¸a˜o Amaro Martins . Valcinir Aloisio Scalla Vulcani . E´verton Lucas de Oliveira . Mariane Barsi Andreeta . Tito Jose´ Bonagamba . Maria Fa´tima Guarizo Klingbeil . Monica Beatriz Mathor . Ana Maria de Guzzi Plepis
Received: 16 December 2019 / Accepted: 26 August 2020 Ó Springer Nature B.V. 2020
Abstract The aim of this study was the development of collagen and collagen/auricular cartilage scaffolds for application in dermal regeneration. Collagen was obtained from bovine tendon by a 72 h-long treatment, while bovine auricular cartilage was treated for 24 h and divided into two parts, external (perichondrium, E) and internal (elastic cartilage, I). The scaffolds were prepared by mixing collagen (C) with the internal part (CI) or the external part (CE) in a 3:1 ratio. Differential scanning calorimetry, scanning electron microscopy
(SEM) analysis, microcomputed tomography imaging (micro-CT) and swelling degree were used to characterize the scaffolds. Cytotoxicity, cell adhesion, and cell proliferation assays were performed using the cell line NIH/3T3. All samples presented a similar denaturation temperature (Td) around 48 °C, while CE presented a second Td at 51.2 °C. SEM micrographs showed superficial pores in all scaffolds and micro-CT exhibited interconnected pore spaces with porosity above 60% (sizes between 47 and 149 lm). The order
L. C. Massimino (&) A. M. de Guzzi Plepis Interunit Graduate Program in Bioengineering, University of Sa˜o Paulo, Sa˜o Carlos, SP, Brazil e-mail: [email protected]
T. J. Bonagamba e-mail: [email protected]
A. M. de Guzzi Plepis e-mail: [email protected] V. da Conceic¸a˜o Amaro Martins A. M. de Guzzi Plepis Sa˜o Carlos Institute of Chemistry, University of Sa˜o Paulo, Sa˜o Carlos, SP, Brazil e-mail: [email protected]
M. F. G. Klingbeil M. B. Mathor Nuclear and Energy Research Institute (IPEN-CNEN/SP), Sa˜o Paulo, Brazil e-mail: [email protected] M. B. Mathor e-mail: [email protected]
V. A. S. Vulcani Anatomy Laboratory, Veterinary Medicine, Federal University of Goia´s, Jataı´, GO, Brazil e-mail: [email protected] E´. L. de Oliveira M. B. Andreeta T. J. Bonagamba Sa˜o Carlos Institute of Physics, University of Sa˜o Paulo, Sa˜o Carlos, SP, Brazil e-mail: [email protected] M. B. Andreeta e-mail: [email protected]
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Cell Tissue Bank
of swelling was CE \ CI \ C and the scaffolds did not present cytotoxicity, showing attachment rates above 75%—all samples showed a similar pattern of proliferation until 168 h, whereas CI tended to decrease after this time. The scaffolds were easily obtained, biocompatible and had adequate morphology for cell growth. All samples showed high adhesion, whereas collagen-only and collagen/external part scaffolds presented a better cell proliferation rate and would be indicated for possible use in dermal regeneration. Keywords Biop
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