Vitamin B12-loaded polycaprolacton/gelatin nanofibrous scaffold as potential wound care material
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ORIGINAL ARTICLE
Vitamin B12-loaded polycaprolacton/gelatin nanofibrous scaffold as potential wound care material Sayeed Farzanfar1 · Gholamreza Savari kouzekonan2 · Ruhollah Mirjani3 · Babak Shekarchi1 Received: 29 February 2020 / Revised: 15 July 2020 / Accepted: 20 July 2020 © Korean Society of Medical and Biological Engineering 2020
Abstract The current study aimed to develop a potential wound dressing using vitamin B12-loaded polycaprolacton/gelatin nanofibrous scaffold. In order to produce wound dressings, 1000 mcg of vitamin B12 was added to polycaprolacton/gelatin solution and the nanofibrous scaffolds were fabricated through electrospinning method. The obtained scaffolds were studied regarding their hydrophobicity, microstructure, amount of water absorption, water vapor permeability, tensile strength, release test, and cellular proliferation assay. In vitro studies revealed that the incorporation of vitamin b12 into polycaprolacton/gelatin scaffolds could significantly augment L929 cells proliferation at 1 and 3 days post-seeding. However, there was not statistically significant difference between Vitamin B12-containing and polymer-only scaffolds in tensile strength study, surface wettability measurement, water vapor transmission test, the capacity for water absorption, and nanofiber’s diameter. Both vitamin containing and free dressings were applied on the full-thickness excisional wound in rat model to compare their healing potential. Our results showed that after 14 days, vitamin B12 containing dressing could significantly enhance wound closure compared to vitamin B12 free scaffolds (92.27 ± 6.84% vs. 64.62 ± 2.96%). Furthermore, histopathological examinations showed significantly greater epithelial thickness in polycaprolacton/gelatin/vitamin B12 group compared to other experimental groups. This preliminary study suggest potential applicability of the proposed dressing to treat skin wounds in clinic. Keywords Vitamin B12 · Electrospinning · Polycaprolacton · Gelatin · Wound dressing
1 Introduction Skin as the largest organ in the body plays a fundamental role in body hemostasis. It serves as the first line of defense against environmental hazards, the source for vitamin D production, and protects body against dehydration, and temperature fluctuations [1]. Although this tissue has an inherent capacity to repair following injury, in case of extensive skin injuries body mechanisms fail to replace the damaged tissue and the wound turns into either chronic non-healing wound or fibrosis tissue [2]. The healing of * Babak Shekarchi [email protected] 1
AJA Radiation Sciences Research Center (ARSRC), Tehran, Iran
2
Department of Medical Nanotechnology, Islamic Azad University, Tehran, Iran
3
Department of Genetics and Advanced Medical Technology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
skin wounds involves series of interrelated phases including inflammation, proliferation, maturation, and remodeling. In chronic wounds, a persisting inflammatory environme
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