Working with Stem Cells
The content covered encompass not only the theoretic and methodological aspects for culturing human pluripotent stem cells, but also the establishment of induced pluripotent stem cells and their differentiation into specific cellular subtypes. This book h
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king with Stem Cells
Working with Stem Cells
Henning Ulrich • Priscilla Davidson Negraes
Working with Stem Cells A Quick and Easy Approach of Methodologies and Applications
Henning Ulrich Department of Biochemistry Institute of Chemistry University of Sao Paulo São Paulo, SP, Brazil
Priscilla Davidson Negraes Department of Biochemistry Institute of Chemistry University of São Paulo São Paulo, SP, Brazil
ISBN 978-3-319-30580-6 ISBN 978-3-319-30582-0 DOI 10.1007/978-3-319-30582-0
(eBook)
Library of Congress Control Number: 2016945374 © Springer International Publishing Switzerland 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer International Publishing AG Switzerland
Preface
We are in a unique moment in human history where stem cells, previously viewed as a great promise in medicine, are now slowly moving into clinical trials. Scientists are now confident to start testing different stem cell-based protocols in humans. For the first time, we will be the pioneers, having the opportunity to do it right, setting the example for the generations to come. Contrary to conventional medicine, cellular therapies will replace damaged tissues and stay there for the lifespan of a person. Alternatively, stem cells can be used as a tool to recapitulate human development and inform us about the disease pathology, proving opportunities for novel diagnostics and therapies. This is an extraordinary example of plasticity, never seen before in medical history. The fundamental aspects of stem cells come back to the methods that were developed over the years to reach to this point. We have learnt how to isolate, grow, enrich, and differentiate stem cells. These methods are still evolving in the scientific laboratories all over the world and will be optimized for the different medical strategies. This book summarizes the most current and important methods, and state-of-the art
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