Yeast Surface Display Methods, Protocols, and Applications
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applic
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Bin Liu Editor
Yeast Surface Display Methods, Protocols, and Applications
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Yeast Surface Display Methods, Protocols, and Applications
Edited by
Bin Liu UCSF, San Francisco, CA, USA
Editor Bin Liu UCSF San Francisco, CA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2747-0 ISBN 978-1-4939-2748-7 (eBook) DOI 10.1007/978-1-4939-2748-7 Library of Congress Control Number: 2015937748 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: The front cover photo was contributed by Maxwell Cherf and Dr. Jennifer R. Cochran. The scanning electron microscope image of yeast cells was purchased from PSmicrographs. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Yeast surface display techniques have evolved into a versatile tool for research and industrial applications. In 1993, Maarten P. Schreuder and colleagues described yeast cell wall display of heterologous proteins. In 1997, K. Dane Wittrup and colleagues described yeast surface polypeptide display library construction and screening. Since then, the field has seen explosive growth. Yeast surface display is now widely used for antibody library screening, protein and antibody engineering, and discovery of novel protein-ligand interactions. Billionmember antibody fragment libraries have been created and displayed on the surface of yeast, and used for identification of novel antibodies with desired affinity and specificity. The technique is also widely used in protein engineering to improve catalytic activities of enzymes, affinity
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