Yeast Cytokinesis Methods and Protocols
This volume presents protocols on yeast cytokinesis that will help Molecular and Cellular Biology researchers in the use of these microorganisms to approach the study of general or specific key questions in cytokinesis. Written for the Methods in Molecula
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Alberto Sanchez-Diaz Pilar Perez Editors
Yeast Cytokinesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Yeast Cytokinesis Methods and Protocols
Edited by
Alberto Sanchez-Diaz Instituto de Biomedicina y Biotecnología de Cantabria, CSIC, Universidad de Cantabria, Santander, Spain; Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain
Pilar Perez Instituto de Biología Funcional y Genómica, CSIC, Universidad de Salamanca, Salamanca, Spain
Editors Alberto Sanchez-Diaz Instituto de Biomedicina y Biotecnología de Cantabria CSIC, Universidad de Cantabria Santander, Spain
Pilar Perez Instituto de Biología Funcional y Genómica CSIC, Universidad de Salamanca Salamanca, Spain
Departamento de Biología Molecular Facultad de Medicina, Universidad de Cantabria Santander, Spain
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3144-6 ISBN 978-1-4939-3145-3 (eBook) DOI 10.1007/978-1-4939-3145-3 Library of Congress Control Number: 2015947262 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The final stage of the eukaryotic cell cycle is cytokinesis, when a mother cell is separated into two cells by a contractile actomyosin ring. This contractile ring is normally assembled in the middle of the cell and is linked to the plasma membrane such that, when it constricts, it creates a cleavage furrow that partitions the cell in two. The process is conserved between fungi and animals. Defects in the placement, timing, assembly, and contraction
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