A Low-Cost and High Sensitive Paper-Based Microfluidic Device for Rapid Detection of Glucose in Fruit
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A Low-Cost and High Sensitive Paper-Based Microfluidic Device for Rapid Detection of Glucose in Fruit Zexian Li 1,2,3 & Yongheng Zhu 1,2,3 & Weijia Zhang 1,2,3 & Changhua Xu 1,2,3 & Yingjie Pan 1,2,3 & Yong Zhao 1,2,3
Received: 11 May 2016 / Accepted: 8 August 2016 # Springer Science+Business Media New York 2016
Abstract The concentration of glucose in fruit is a key index for fruit quality. Despite the fact that there are many methods to detect glucose, finding a rapid, visual, and inexpensive method to detect glucose in fruit is still a great challenge. In this study, the concept of paper-based microfluidic device (μPAD) was extended to high-performance chemo-sensors for semi-quantitative detection of glucose in different kinds of fruit. Under optimized conditions, the results showed that the method detected glucose of different concentrations and the limit of detection (LOD) can reach 3.12 mM. In the quantifications, a good linear relationship was obtained between mean intensity and glucose concentration (5∼50 mM, R2 = 0.952). The analytical results obtained by the developed approach were in good agreement with the results by spectrophotometric method. In addition, polyethylene glycol (PEG) was added to cellulose paper surface to improve the both hydrophilic and sensing performance, indicating that the PEG-cellulose paper was superior for its rapid response for glucose. Herein, a low-cost and high sensitive μPAD sensor was exploited to assess the fruit quality and guide fruit intake for diabetes mellitus patients.
* Yongheng Zhu [email protected] * Yong Zhao [email protected] 1
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2
Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China
3
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China
Keywords Paper-based microfluidic device (μPAD) . Glucose . Fruit
Introduction Fruit contains many kinds of sugars such as glucose, fructose, and sucrose, which are related to perceived sweetness and may be used as an index of consumer acceptability (Jawaheer et al. 2003). Glucose is a major monosaccharide in almost all fruit and plays an important role in the evaluation of fruit quality (Liu et al. 2006). Glucose intake is predicted an increase in blood glucose level, while excessive high-glucose fruit intake responds sensitively to glycemic load for diabetes mellitus patients (Liu et al. 2004; Xi et al. 2014). A more detailed knowledge of glucose concentration in fruit is still needed because it will not only benefit the selection of fruit for high quality of utilization but it is also crucial for diabetics or people who react sensitively to glucose (Ma et al. 2014; Montonen et al. 2007). Thus, there is an obvious need to the development of rapid, portable, and economical glucose sensor for measuring the glucose concentration in fruit, assessing the fruit quality, identifying
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