A sample volume independent paper microfluidic device for quantifying glucose in real human plasma
- PDF / 2,106,551 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 79 Downloads / 160 Views
RESEARCH PAPER
A sample volume independent paper microfluidic device for quantifying glucose in real human plasma Mehrdad Lotfi Choobbari1 · Mohammad Behnam Rad2 · Amir Jahanshahi1 · Hedayatollah Ghourchian2 Received: 1 May 2020 / Accepted: 13 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The uncontrolled volume of sample has a significant influence on the outcome of the colorimetric detection method performed on paper-based microfluidic devices. In a reliable colorimetric assay, the results should be completely dependent on the concentration of analyte in the sample rather than its volume. However, this effect is usually overlooked in the development of paper-based microfluidic devices as a simple, inexpensive, portable and user-friendly medical diagnostic system. In this work, a volume-independent micro paper-based analytical device has been introduced in which the need for precise controlling of sample volume is eliminated. We have shown that the proposed device is only sensitive to the concentration of analyte (glucose) in the sample instead of its volume. We have also demonstrated that the proposed device addresses the reported problem of colour heterogeneity and is relatively insusceptible to the variation of reagents volume. An inexpensive smart phone camera is used for capturing the images and a portable 3D printed tool is designed to eliminate the environmental illuminations. To simulate the real condition, human plasma samples are used throughout the experiments. Keywords Paper microfluidic · Bio-sensor · Colorimetry · Glucose · Sample volume
1 Introduction The prevalence of diabetes has been steadily increasing over the last decades, as a consequence of modern lifestyle. Early diagnosis is a practical approach to manage and control this non-communicable disease and prevent its progress (Organization 2016). This and similar works are targeted towards a portable inexpensive platform for early diagnosis of diabetes. Paper-based microfluidics is an emerging technology that could theoretically prevail in low-cost detection of
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10404-020-02382-y) contains supplementary material, which is available to authorized users. * Amir Jahanshahi [email protected] * Hedayatollah Ghourchian [email protected] 1
Micro Bio Technology Laboratory (MBTechLab), Department of Electrical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
Laboratory of Bio‑Analysis, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
2
glucose. Nevertheless, there are still challenges to overcome (Yamada et al. 2017). Micropaper-based analytical devices (μPADs) have been introduced as a relatively small, inexpensive, portable and disposable technology in 2007 by Whitesides group (Martinez et al. 2007). Practical applications of μPADs are mainly concentrated for medical diagnostics, i.e., glucose (Martinez et al. 2008; Gabriel et al.
Data Loading...
