A nanocomposite consisting of cuprous oxide supported on graphitic carbon nitride nanosheets for non-enzymatic electroch
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ORIGINAL PAPER
A nanocomposite consisting of cuprous oxide supported on graphitic carbon nitride nanosheets for non-enzymatic electrochemical sensing of 8-hydroxy-2′-deoxyguanosine Umamaheswari Rajaji 1 & Subash Vetri Selvi 1 & Shen-Ming Chen 1 & Sathishkumar Chinnapaiyan 1 & Tse-Wei Chen 1,2,3 & Mani Govindasamy 1,4 Received: 30 November 2019 / Accepted: 26 June 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Graphitic carbon nitrides supported cuprous oxide architecture is reported as an efficient electrode material for supercapacitors, especially due to its high charge-transfer conductivity of the electrochemical devices. Herein, we present an electrochemical sensor to specifically detect 8-hydroxy-2′-deoxyguanosine (8-HDG) oxidative stress biomarker using graphitic carbon nitrides that decorate a cuprous oxide cubes modified electrode. The fabricated electrochemical sensor was characterized and proved by electrochemical methods, EDX, FESEM, and amperometry (i–t). In the presence of 8-hydroxy-2′-deoxyguanosine (8-HDG), the effective interaction between graphitic carbon nitrides and 8-HDG favors the accumulation on the Cu2O/g-C3N4/GCE, which increases the electrocatalytic property and amperometric response. The proposed electrochemical sensor exhibits a wide linear range for 8-HDG in 0.1 M phosphate buffer (pH 7.0) from 25 nM to 0.91 mM, and the limit of detection (LOD) is 4.5 nM. The stability of the Cu2O/g-C3N4/GCE is improved when stored at 4 °C. The repeatability and reproducibility of this electrochemical sensor is good and the sensor retains its current response for 8-HDG detection also after long time storage. The modified sensor proved high selectivity and sensitivity for 8HDG, which made it possible to determine 8-HDG in biological samples. Furthermore, the Cu2O/g-C3N4/GCE offered a favorable electron transfer between the Cu2O/g-C3N4 and the electrode interface compared to Cu2O/GCE, g-C3N4/GCE, and unmodified GCE. Keywords 8-hydroxy-2′-deoxyguanosine . Oxidative stress marker . Electrocatalyst . Sonochemical approach . Electrochemical detection
Introduction Reactive oxygen species (ROS) and free radicals were also formed as produced in mankind are balanced at an
optimum level by the antioxidant system of our body [1–3]. The oxidative stress is a situation that leads to oxidative imbalance due to the excessive production of free radicals. The ROS content in our body produces
Umamaheswari Rajaji and Subash Vetri Selvi contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04416-2) contains supplementary material, which is available to authorized users. * Shen-Ming Chen [email protected] * Mani Govindasamy [email protected] 1
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, Republic of China
2
Research and Development Center for Smart Textile Technology, National Taipe
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