A new storage solution for the hypothermic preservation of corneal grafts: an experimental study
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A new storage solution for the hypothermic preservation of corneal grafts: an experimental study Ismini Koulouri . Olaf Hellwinkel . Sibylle Altena¨hr . Martin Spitzer . Stefan Fritz . Jana Feuerstacke . Filip Filev
Received: 5 November 2019 / Accepted: 15 May 2020 Ó Springer Nature B.V. 2020
Abstract In this experimental study we used for the first time TiprotecÒ as a solution for corneal preservation and cold storage. We compared the resultant endothelial cell morphology and viability with this obtained after preservation of the ex-vivo corneas with both usual standard techniques: conventional cold storage (using Eusol-C) and organ culture. This prospective, in vitro, 3-armed parallel study was performed with the use of 90 porcine corneas (examined for their endothelial quality and transparency) randomly selected for preservation in three storage methods (each 30 corneas): organ culture, standard cold storage (Eusol-C) and experimental cold storage (TiprotecÒ) Endothelium cell quantity and quality as Ismini Koulouri and Olaf Hellwinkel author contributed equally to this paper
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10561-020-09838-z) contains supplementary material, which is available to authorized users. I. Koulouri O. Hellwinkel S. Altena¨hr Department of Forensic Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany I. Koulouri Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
well as corneal opacification were assessed. The degree of endothelial transparency was significantly reduced over time with all preservation media, without any significant difference among the three groups at any point of time. A reduction in endothelial cell density was also observed with all three preservation media after 30 days of storage without statistically significant differences between groups. The number of hexagonal and pentagonal endothelium cells was significantly reduced overtime in all media with significantly more hexagonal and pentagonal in the organ culture group compared to the cold storage groups. We could show that the cryopreservation medium TiprotecÒ, used until now for the preservation of vascular grafts, was of similar quality compared to the medium Eusol-C for the hypothermic storage of corneal tissue for an extended period of time up to 30 days. In comparison to organic culture with culture medium KII, both TiprotecÒ and Eusol-C were found less effective in preserving endothelial cell quality, as
S. Fritz Dr. Franz Ko¨hler Chemie GmbH, Bensheim, Germany F. Filev (&) Werner Forssmann Clinic, Rudolf-Breitscheid-Straße 100, 16225 Eberswalde, Germany e-mail: [email protected]
M. Spitzer J. Feuerstacke F. Filev Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
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Cell Tissue Bank
assessed by the morphometric analysis, and viability, as assessed by the degree of vacuolization at least up to the 30th day of storage. However, both,
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