A new trend in the medication of hepatocyte cytoxicity in mice: protective role of probiotic bacteria
- PDF / 1,371,282 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 68 Downloads / 164 Views
RESEARCH ARTICLE
A new trend in the medication of hepatocyte cytoxicity in mice: protective role of probiotic bacteria Nanis G. Allam 1 & Mostafa M. El-Sheekh 1
&
Naglaa I. Sarhan 2 & Ghada S. Alfakharany 1
Received: 6 April 2020 / Accepted: 6 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Liver toxicity is affected by several factors, including certain medications, fumes emission from factories, materials used in industries, and exposure to chemicals such as carbon tetrachloride (CCl4). Some preselected probiotic bacteria strains have been widely employed in different medical researches due to their antioxidant, anticancer, antimicrobial, anti-inflammatory characters, and hepatoprotective factor. The present study was aimed to evaluate the protective role of probiotic bacteria (Lactobacillus plantarum DSMZ 20174) and their ameliorative effects against CCl4-induced hepatotoxicity in mice. The cell cycle of hepatocytes and the expression of transforming growth factor-β (TGF-β) were assessed by flow cytometry as indicators for apoptosis. The antioxidant activity of probiotic bacteria was estimated by measuring lipid peroxidation (LPO) and scavenging 2,2-diphenyl1-picryl-hydrazyl (DPPH). The results showed that the treatment of CCl4-administered mice by supernatant from Lactobacillus plantarum DSMZ 20174 induced an amelioration in CCl4-induced increases in serum activity of the liver enzymes and decreases in LPO and DPPH. After treatment with probiotics, the liver histopathological studies showed abundant infiltration and accumulation of mononuclear cells and fibroblast, indicating a positive effect ameliorating the damage previously induced by CCl4. In sum, the results of the present work indicate the protective effects of Lactobacillus plantarum against hepatotoxicity through antioxidant effects. Keywords Antioxidant . Biotherapy . CCl4 . Hepatoprotective . Lactobacillus plantarum subsp. plantarum DSMZ 2017
Introduction The liver is an essential organ in the human body, due to its vital functions, including glycogen storage, plasma protein synthesis, decomposition of red blood cells, and detoxification (Opoku et al. 2007). The liver plays the main role in the metabolism and excretion of xenobiotics, which makes it extremely sensitive to their toxic effects. Liver damage by various toxic chemicals or their reacting metabolites (hepatotoxicants) is known as hepatotoxicity Singh et al. (2014). The mechanisms behind how certain drugs or Responsible Editor: Mohamed M. Abdel-Daim * Mostafa M. El-Sheekh [email protected] 1
Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
2
Histology Department, Faculty of Medicine, Tanta University, Tanta, Egypt
chemicals induce liver damage are mostly related to oxidative stress and a cellular imbalance between the production and removal of free radicals (Castro and Freeman 2001). Overproduction of free radicals directly impairs hepatocyte membrane by lipid peroxidation, followed by a chain of cellu
Data Loading...
