A newly isolated green alga Chlorella sp. KLSc59: potential for biohydrogen production
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A newly isolated green alga Chlorella sp. KLSc59: potential for biohydrogen production Thanaporn Sirawattanamongkol 1 & Thanaporn Maswanna 2 & Cherdsak Maneeruttanarungroj 3,4 Received: 29 January 2020 / Revised and accepted: 27 April 2020 # Springer Nature B.V. 2020
Abstract Hydrogen production from microalgae has attracted considerable attention due to its high energy content and as a renewable and environmentally friendly energy source. Various strains of microalgae have been reported to produce “biohydrogen”, but screening for new strains is still necessary to discover strains with higher hydrogen yields. A newly isolated hydrogenproducing green alga was screened and labeled Chlorella sp. KLSc59. The effect of extracellular pH, light intensity, external carbon sources, reducing agents, and nutrient deprivation on biohydrogen production of Chlorella sp. KLSc59 were investigated. Hydrogen yield was higher under anaerobic conditions. Under external pH 7.2 with 53.2 μmol photons m−2 s−1 light intensity and using acetate as a carbon source, the optimum hydrogen yield was 281 μmol H2 mg−1 Chl. Nutrient deprivation reduced the hydrogen yield. Several reducing agents were assessed, and 1 mM ethanol enhanced yield by 3 times for 850 μmol H2 mg−1 Chl, and 1 mM sodium dithionite increased yield by 2.7 times for 750 μmol H2 mg−1 Chl. Significantly, our new strain showed higher hydrogen yields ranging from 1.5 to 68 times compared with other microalgae. Thus, Chlorella sp. KLSc59 showed valuable potential for biohydrogen production. Keywords Chlorophyta . Biohydrogen . Yield optimization . Chlorella sp. KLSc59
Introduction Hydrogen has been shown as potential energy source for replacing fossil fuels since this outstanding energy carrier contains a high energy density, 144 MJ kg−1 (Lorencini et al. 2016), while its combustion produces only water. Biohydrogen production by microorganisms, such as bacteria, cyanobacteria, and algae, have shown great promise for * Cherdsak Maneeruttanarungroj [email protected] Thanaporn Sirawattanamongkol [email protected] Thanaporn Maswanna [email protected] 1
Department of Chemistry, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
2
Scientific Instrument Center, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
3
Department of Biology, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
4
Bioenergy Research Unit, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand
generating large-scale sustainable energy due to its use of cheap and renewable energy source, including water and sunlight. Microalgae, including green algae and cyanobacteria, can produce biohydrogen, via biophotolysis (Kosourov et al. 2007; Allakhverdiev et al. 2010; Maneeruttanarungroj et al. 2010; Maswanna et al. 2018; Khosravitabar 2020). The process extracts electrons and protons from water and transduces electrons via ferred
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