A Novel Galactoglucomannan Exopolysaccharide Produced by Oil Fermentation with Pseudozyma sp. SY16
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pISSN 1226-8372 eISSN 1976-3816
RESEARCH PAPER
A Novel Galactoglucomannan Exopolysaccharide Produced by Oil Fermentation with Pseudozyma sp. SY16 Kwang Nyeon Kim, Yong Jae Lee, Dae-Hyun Cho, Keug-Hyun Ahn, Jong-Eon Hong, Yong-Il Park, and Hee-Sik Kim
Received: 2 March 2020 / Revised: 20 March 2020 / Accepted: 22 March 2020 © The Korean Society for Biotechnology and Bioengineering and Springer 2020
Abstract Recently, the production of exopolysaccharide from Pseudozyma sp. SY16, which is mannosylerythritol lipid-producing yeast, has been occasionally observed during industrial fermentations. Moreover, exopolysaccharides derived from microbes are in high demand for the production of biopolymers. Therefore, this study aimed to investigate the production and characteristics of the exopolysaccharide produced by this strain. In batch fermentations with this strain, the maximum production yield of exopolysaccharide (5.8 g/L) was obtained under olive oil supplementation as a carbon source with culture conditions of 30°C, pH 8.0, and 600 rpm. Through a series of liquid chromatography steps, the exopolysaccharide was successfully purified, and its emulsifying activity was demonstrated. In addition, two-dimensional nuclear magnetic resonance analysis revealed that the exopolysaccharide is a galactoglucomannan with a novel and unique structure, Kwang Nyeon Kim† R&D Center, United Active Inc., Seoul 08381, Korea Yong Jae Lee†, Dae-Hyun Cho, Keug-Hyun Ahn, Hee-Sik Kim Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea Jong-Eon Hong, Hee-Sik Kim DKBio Ltd., Daejeon 34141, Korea Yong-Il Park Department of Biotechnology and The Research Centre for Biopharmaceutical Lead Molecule (GRRC), The Catholic University of Korea, Bucheon 14662, Korea Hee-Sik Kim* Major of Environmental Biotechnology, University of Science & Technology (UST), Daejeon 34113, Korea Tel: +82-42-860-4326; Fax: +82-42-860-4594 E-mail: [email protected] †
Kwang Nyeon Kim and Yong Jae Lee contributed equally to this work
consisting of galactose, glucose, and mannose in the ratio of 1:5:3. Keywords: exopolysaccharide, galactoglucomannan, Pseudozyma sp., yeast, fermentation
1. Introduction Pseudozyma sp. SY16 is a previously isolated yeast strain that has since been used for the commercial production of a biosurfactant, mannosylerythritol lipid (MEL), which exhibits high emulsifying activity and interfacial tension reduction [1]. During industrial fermentation, the potent production of exopolysaccharide (EPS) has been occasionally observed from this strain. EPS derived from biological resources are in high demand for the production of biopolymers. Accordingly, there is substantial interest in the isolation and identification of new microbial polysaccharides with high potential for industrial applications, such as gelling agents, emulsifiers, and flocculants [2-5]; stabilizers and texture-enhancing agents [4,6]; and potent antioxidants and anti-inflammatory agents. However, the production of EPS thr
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