Anti-Proliferative and Anti-Biofilm Potentials of Bacteriocins Produced by Non-Pathogenic Enterococcus sp.
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Anti-Proliferative and Anti-Biofilm Potentials of Bacteriocins Produced by Non-Pathogenic Enterococcus sp. Fatma Molham 1 & Ahmed S. Khairalla 1,2 Sameh AbdelGhani 1,4
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Ahmed F. Azmy 1
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Eman El-Gebaly 1,3
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Ahmed O. El-Gendy 1
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Accepted: 20 September 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The incidence of cancer is increasing worldwide; likewise, the emergence of antibiotic-resistant biofilm-forming pathogens has led to a tremendous increase in morbidity and mortality. This study aimed to evaluate the probiotic properties of bacteriocinproducing Enterococcus sp. with a focus on their anti-biofilm and anticancer activities. Three of 79 Enterococcus isolates (FM43, FM65, FM50) were identified as producers of broad-spectrum bioactive molecules and were molecularly characterized as Enterococcus faecium by 16S rRNA sequencing. Phenotypic and genotypic screening for potential virulence factors revealed no factors known to promote pathogenicity. Treatment with proteinase K resulted in diminished antimicrobial activity; PCRbased screening for bacteriocin genes suggested the presence of both entA and entB genes that encode enterocins A and B, respectively. Maximum antimicrobial activity was detected during the early stationary phase, while activity disappeared after 24 h in culture. Bacteriocins from these isolates were stable at high temperatures and over a wide range of pH. Interestingly, crude supernatants of Ent. faecium FM43 and Ent. faecium FM50 resulted in significant destruction (80% and 48%, respectively; P < 0.05) of Streptococcus mutans ATCC 25175–associated preformed biofilms. Moreover, in vitro cytotoxicity assays revealed that extracts from Ent. faecium isolates FM43, FM65, and FM50 inhibited Caco-2 cell proliferation by 76.9%, 70%, and 85.3%, respectively. Taken together, the multifunctional capabilities of the microbial-derived proteins identified in our study suggest potentially important roles as alternative treatments for biofilm-associated infections and cancer. Keywords Bacteriocin . Biofilm . Caco-2 cancer cell line . Enterococcus . Pathogenicity . Streptococcus mutans
Introduction Ahmed S. Khairalla equally contributed as the first author Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12602-020-09711-1) contains supplementary material, which is available to authorized users. * Ahmed F. Azmy [email protected] * Ahmed O. El-Gendy [email protected] 1
Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
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Department of Biology, University of Regina, Regina, Saskatchewan, Canada
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Department of Microbiology and Immunology, Faculty of Pharmacy, October 6 University, Cairo 12585, Egypt
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Department of Pathology & Medical Laboratory, School of Medicine, University of Louisville, Louisville, KY, USA
Enterococcus is one of the main genera belonging to the lactic acid bacteria (LAB) group. Pathogenic and commensal strains
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