Sphingobacterium endophyticum sp. nov., a novel endophyte isolated from halophyte
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ORIGINAL PAPER
Sphingobacterium endophyticum sp. nov., a novel endophyte isolated from halophyte Yang‑Yang Liu1 · Fang Liu1 · Yu‑Qian Li2 · Rui‑Feng Lei1 · Qin Ma1 · Manik Prabhu Narsing Rao3 · Dilireba Abudourousuli1 · Zulihumaer Rouzi1 · Muyesaier Aosiman1 · Deng‑Di An1 · Wen‑Jun Li3,4 Received: 7 May 2020 / Revised: 2 July 2020 / Accepted: 24 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A bacterial strain designated NYYP31T was isolated from the leaves of an annual halophytes, Suaeda corniculata Bunge, collected from the southern edge of the Gurbantunggut desert, north-west China. Strain N YYP31T was Gram-staining negative, strictly aerobic, rod-shaped, non-motile, and non-spore-forming. Growth was observed at 4–42 °C, at pH 5.0–10.0, in the presence of up to 8% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and coding sequences of 92 protein clusters showed that strain NYYP31T should be assigned to the genus Sphingobacterium. 16S rRNA gene sequence similarity analysis showed that strain N YYP31T was most closely related to the type strain of Sphingobacterium daejeonense (97.9%) and Sphingobacterium lactis (97.7%). The predominant isoprenoid quinone was MK-7. The major fatty acids were identified as iso-C15:0, iso-C17:0 3-OH and summed feature 3 ( C16:1 ω7c and/or C 16:1 ω6c). The polar lipids were phosphatidylethanolamine, two unidentified phospholipids, three unidentified lipids, three unidentified amino phospholipids, and two unidentified glycolipids. The genomic DNA G + C content was 36.4 mol%. The average nucleotide identity (ANI) values for strain NYYP31T to the type strains of S. daejeonense and S. lactis were 77.9 and 74.1%, respectively, which were below the cut-off level (95–96%) for species delineation. Based on the above results, strain N YYP31T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium endophyticum sp. nov. is proposed. The type strain is NYYP31T (= CGMCC 1.16979T = NBRC 114258T). Keywords Sphingobacterium endophyticum · Halophytes · Genome analysis · Polyphasic taxonomy Abbreviations ANI Average nucleotide identity dDDH Digital DNA–DNA hybridization
ML Maximum-likelihood NJ Neighbor-joining UBCG Up-to-date bacterial core gene set
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02000-z) contains supplementary material, which is available to authorized users. * Deng‑Di An [email protected] * Wen‑Jun Li [email protected] 1
Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi 830054, People’s Republic of China
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361102, People’s Republic of China
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State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, Scho
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