A Novel Paper-plastic Microfluidic Hybrid Chip Integrated with a Lateral Flow Immunoassay for Dengue Nonstructural Prote
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Original Article
A Novel Paper-plastic Microfluidic Hybrid Chip Integrated with a Lateral Flow Immunoassay for Dengue Nonstructural Protein 1 Antigen Detection Ma. Kristine Yuzon, Jae-Heon Kim & Sanghyo Kim
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Received: 20 April, 2019 / Accepted: 18 July, 2019 / Published online: 11 September, 2019 ⒸThe Korean BioChip Society and Springer 2019
Abstract This study presents an easy paper-plastic hybrid microfluidic chip fabrication method that explored the use of oven baking a wax-printed pattern for microfluidic channel assembly combined with the use of a double-sided adhesive tape for layer binding. More specifically, the method includes printing of a wax pattern onto the CA film & oven baking of the pattern along with the backed NC membrane at 120°C for 2.5 minutes. The porous nature of the NC membrane allows the wax pattern to enter into its pores during the baking process and binds the backed NC membrane with the CA film. Attachment of the engraved PMMA layer was performed to increase the structural support. The engraved hole of the layer allows the conjugate pad with space for its integration into the system. Moreover, the antibodies required for the integration of the chip with the LFIA were selected by enzyme-linked immunosorbent assay (ELISA). A CA film, an NC membrane, and a PMMA sheet were used to develop a microfluidic chip that can be integrated with a sandwich format lateral flow immunoassay used to detect dengue non-structural 1 protein. The fabricated chip was able to successfully detect a concentration of dengue non-structural 1 protein of at least 84.66 ng/mL. Keywords: Microfluidic chip, Lateral flow immunoassay, Colorimetric detection, Dengue Department of Bionanotechnology, Gachon University, 1342 Seongnamdaero, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 13120, Republic of Korea *Correspondence and requests for materials should be addressed to S.H. Kim ( [email protected])
Introduction The concept of microfluidic paper-based devices (μPADs) has been studied extensively over the past few years due to its cheap, portable, disposable, and user-friendly features. As part of the point-of-care testing (POCT) technology, μPADs are being studied in the detection of viruses1 and bacteria2, proteins3 and nucleic acids4, and other chemical compounds5,6. In addition, the incorporation of an assay into a miniaturized chip entails several advantages such as lesser sample and reagent volume, faster reaction time, and higher integration capabilities7-9. Moreover, in a microfluidic chip containing a hydrophilic channel surface and a hydrophobic channel wall, the need for valves and pumps are eliminated due to the possible exploitation of the automatic capillary flow mechanism10. Dengue virus (DENV), a mosquito-borne virus and a member of the Flaviviridae family and of the genus Flavivirus, is comprised of four serotypes, DENV1 to DENV411. An infection of DENV can lead to dengue. Most areas with prevalent dengue cases run to conventional methods of detecting dengue. However, these are time consuming, tedious, expe
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