A preliminary study on the development of a novel biomatrix by decellularization of bovine spinal meninges for tissue en
- PDF / 2,634,876 Bytes
- 14 Pages / 547.087 x 737.008 pts Page_size
- 64 Downloads / 163 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
A preliminary study on the development of a novel biomatrix by decellularization of bovine spinal meninges for tissue engineering applications Eren Ozudogru . Yavuz Emre Arslan
Received: 28 February 2020 / Accepted: 25 August 2020 Ó Springer Nature B.V. 2020
Abstract Here, we aim at developing a novel biomatrix from decellularized bovine spinal meninges for tissue engineering and regenerative medicine applications. Within this concept, the bovine spinal meninges were decellularized using 1% Triton X-100 for 48 h, and residual nuclear content was determined with double-strand DNA content analysis and agarose gel electrophoresis. The major matrix components such as sulfated GAGs and collagen before and after the decellularization process were analyzed with DMMB, hydroxyproline assay and SDS-PAGE. Subsequently, the native bovine spinal meninges (nBSM) and decellularized BSM (dBSM) were physiochemically characterized via ATR-FTIR spectroscopy, TGA, DMA and tensile strength test. The dsDNA content in the nBSM was 153.39 ± 53.93 ng/mg dry weight, versus in the dBSM was 39.47 ± 4.93 ng/mg (n = 3) dry weight and DNA fragments of more than 200 bp in length were not detected in the dBSM by agarose gel electrophoresis. The sulfated GAGs contents for nBSM and dBSM were observed to be 10.87 ± 1.2 and 11.42 ± 2.01 lg/mg dry weight, respectively. The maximum strength of dBSM in dry and wet conditions was found to be 19.67 ± 0.21 MPa and 13.97 ± 0.17 MPa, while
E. Ozudogru Y. E. Arslan (&) Regenerative Biomaterials Laboratory, Department of Bioengineering, Engineering Faculty, Canakkale Onsekiz Mart University, Canakkale 17100, Turkey e-mail: [email protected]
nBSM (dry) was found to be 26.26 ± 0.28 MPa. MTT, SEM, and histology results exhibited that the cells attached to the surface of dBSM, and proliferated on the dBSM. In conclusion, the in vitro preliminary study has demonstrated that the dBSM might be a proper and new bioscaffold for tissue engineering and regenerative medicine applications. Keywords Bovine spinal meninges Decellularization Biomatrix Human adiposederived mesenchymal stem cell Tissue engineering
Introduction Tissue engineering and regenerative medicine, which involve the combination of biology, chemistry, medicine, and engineering, are multidisciplinary realms that are rapidly expanding. For the reconstruction of tissues and organs that entirely and/or partially lost its function, these fields offer alternative approaches such as constructing sophisticated substitutes (i.e., bioscaffolds) of native tissues (Sicari et al. 2014; Erten and Arslan 2018). According to the European Society for Biomaterials (ESB), biomaterials can interface with biological systems in case of tissue deficiencies and support, heal, replace, or reestablish standard bodily functions (O’Brien 2011; Chen and Liu 2016). Biopolymers that are used for the
123
Cell Tissue Bank
reconstruction of tissues or organs can be classified as natural (e.g., collagen, cellulose, decellular
Data Loading...
