Genomic characterization of a novel bovine papillomavirus type 28
- PDF / 1,202,772 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 98 Downloads / 178 Views
ORIGINAL PAPER
Genomic characterization of a novel bovine papillomavirus type 28 Nanako Yamashita‑Kawanishi1 · Masano Tsuzuki1 · Fumi Kasuya1 · Hui‑Wen Chang2 · Takeshi Haga1 Received: 3 March 2020 / Accepted: 28 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Infection of bovine papillomavirus (BPV) has been associated with mucosal and/or cutaneous tumor development in bovids. To date, up to 27 genotypes of BPVs have been identified and classified based on the nucleotide sequence identity of L1 open reading frame. In the present study, the complete sequence of a novel BPV concurrently identified with BPV1 and BPV2 in the facial cutaneous papilloma lesion of a domestic cattle was characterized. The whole genome of the unclassified BPV was 7263 base pairs in full length with GC ratio of 42.9%. In comparison with published BPV sequences, L1 nucleotide sequence of the novel BPV shared 75% identity with BPV15, and was suggested to be classified in the genus, Xipapillomavirus. According to the criteria established by the International Committee on the Taxonomy of Viruses, the novel BPV was designated as BPV type 28. Keywords Bovine papillomavirus · Xipapillomavirus · BPV28 · Phylogenetic analysis · Sequence analysis
Introduction Papillomavirus (PV) is a non-enveloped double-stranded DNA virus, known to be associated with cutaneous and/ or mucosal neoplastic diseases in humans and various animals. The virus comprises five to eight early region open reading frames (ORFs), two late region (L) ORFs, and a non-coding region, around the genomic size about 7000 base pairs (bp) [19]. Based on the criteria established by the International Committee on the Taxonomy of Viruses (ICTV), PVs are classified based on the nucleotide sequence identity of the major capsid protein, L1 ORF [22]. The first PV genotype identified in domestic cattle, bovine (Bos taurus) Edited by Takeshi Noda. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11262-020-01779-9) contains supplementary material, which is available to authorized users. * Takeshi Haga [email protected]‑tokyo.ac.jp 1
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan
School of Veterinary Medicine, Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, Taipei, Taiwan
2
papillomavirus type 1 (BPV1) was announced in 1982 [2]. To date, BPVs have the most varieties, up to 27 genotypes, in animal PVs as described in Papillomavirus Episteme (PaVE) [7]. In general, cutaneous papilloma lesions induced by BPV infection are histologically benign and show spontaneous regression [11]. Currently, BPVs are classified into five genera. BPVs classified in the genera, Deltapapillomavirus have a unique feature that show cross-species infection [15]. Thirteen BPV genotypes classified in Xipapillomavirus are suggested to have associatio
Data Loading...
